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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/220608
Regulation of Claspin by the p38 stress-activated protein kinase protects cells from DNA damage
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Stress-activated protein kinases (SAPKs) enhance survival in response to environmental changes. In yeast, the Hog1 SAPK and Mrc1, a protein required for DNA replication, define a safeguard mechanism that allows eukaryotic cells to prevent genomic instability upon stress during S-phase. Here we show that, in mammals, the p38 SAPK and Claspin-the functional homolog of Mrc1-protect cells from DNA damage upon osmostress during S-phase. We demonstrate that p38 phosphorylates Claspin and either the mutation of the p38-phosphorylation sites in Claspin or p38 inhibition suppresses the protective role of Claspin on DNA damage. In addition, wild-type Claspin but not the p38-unphosphorylatable mutant has a protective effect on cell survival in response to cisplatin treatment. These findings reveal a role of Claspin in response to chemotherapeutic drugs. Thus, this pathway protects S-phase integrity from different insults and it is conserved from yeast to mammals.
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ULSAMER, Arnau, MARTÍNEZ LIMÓN, Adrián, BADER, S, RODRÍGUEZ ACEBES, Sara, FREIRE, Raimundo, MÉNDEZ, Juan, NADAL CLANCHET, Eulàlia de, POSAS, Francesc. Regulation of Claspin by the p38 stress-activated protein kinase protects cells from DNA damage. _Cell Reports_. 2022. Vol. 40, núm. 12. [consulta: 23 de gener de 2026]. ISSN: 2211-1247. [Disponible a: https://hdl.handle.net/2445/220608]