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2016-11-16

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cc by (c) Sanz et al., 2016
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/104548

The disruption of GDP-fucose de novo biosynthesis suggests the presence of a novel fucose-containing glycoconjugate in Plasmodium asexual blood stages

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http://dx.doi.org/10.1038/srep37230

Resum

Glycosylation is an important posttranslational protein modification in all eukaryotes. Besides glycosylphosphatidylinositol (GPI) anchors and N-glycosylation, O-fucosylation has been recently reported in key sporozoite proteins of the malaria parasite. Previous analyses showed the presence of GDP-fucose (GDP-Fuc), the precursor for all fucosylation reactions, in the blood stages of Plasmodium falciparum. The GDP-Fuc de novo pathway, which requires the action of GDP-mannose 4,6-dehydratase (GMD) and GDP-L-fucose synthase (FS), is conserved in the parasite genome, but the importance of fucose metabolism for the parasite is unknown. To functionally characterize the pathway we generated a PfGMD mutant and analyzed its phenotype. Although the labelling by the fucose-binding Ulex europaeus agglutinin I (UEA-I) was completely abrogated, GDP-Fuc was still detected in the mutant. This unexpected result suggests the presence of an alternative mechanism for maintaining GDP-Fuc in the parasite. Furthermore, PfGMD null mutant exhibited normal growth and invasion rates, revealing that the GDP-Fuc de novo metabolic pathway is not essential for the development in culture of the malaria parasite during the asexual blood stages. Nonetheless, the function of this metabolic route and the GDP-Fuc pool that is generated during this stage may be important for gametocytogenesis and sporogonic development in the mosquito.

Matèries

Malària, Malalties parasitàries

Matèries (anglès)

Malaria, Parasitic diseases

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Articles publicats en revistes (ISGlobal)
<b>Publicacions de projectes de recerca finançats per la UE</b>

Citació

SANZ SENDER, Silvia, LÓPEZ GUTIÉRREZ, Borja, BANDINI, Giulia, DAMEROW, Sebastian, ABSALON, Sabrina, DINGLASAN, Rhoel r., SAMUELSON, John, IZQUIERDO LÁZARO, Luis. The disruption of GDP-fucose de novo biosynthesis suggests the
                presence of a novel fucose-containing glycoconjugate in
                Plasmodium asexual blood stages. _Scientific Reports_. 2016. Vol. 6, núm. 37230. [consulta: 29 de gener de 2026]. ISSN: 2045-2322. [Disponible a: https://hdl.handle.net/2445/104548]

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