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Bacterial cellulose matrices to develop enzymatically active paper
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This work studies the suitability of bacterial cellulose (BC) matrices to prepare enzymatically active nanocomposites, in a framework of more environmentally friendly methodologies. After BC production and purification, two kind of matrices were obtained: BC in aqueous suspension and BC paper. A lipase was immobilised onto the BC matrices by physical adsorption, obtaining Lipase/BC nanocomposites. Neither morphology nor crystallinity, measured by scanning electron microscopy (SEM) and X-Ray diffractometry (XRD) respectively, of the BC were affected by the binding of the protein. The activity of Lipase/BC suspension and Lipase/BC paper was tested under different conditions, and the operational properties of the enzyme were evaluated. A shift towards higher temperatures, a broader pH activity range, and slight differences in the substrate preference were observed in the immobilised lipase, compared with the free enzyme. Specific activity was higher for Lipase/BC suspension (4.2 U/mg) than for Lipase/BC paper (1.7 U/mg) nanocomposites. However, Lipase/BC paper nanocomposites showed improved thermal stability, reusability, and durability. Enzyme immobilised onto BC paper retained 60% of its activity after 48 h at 60 ºC. It maintained 100% of the original activity after being recycled 10 times at pH 7 at 60 ºC and it remained active after being stored for more than a month at room temperature. The results suggested that lipase/BC nanocomposites are promising biomaterials for the development of green biotechnological devices with potential application in industrials bioprocesses of detergents and food industry and biomedicine. Lipase/BC paper nanocomposite might be a key component of bioactive paper for developing simple, handheld, and disposable devices.
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BURUAGA RAMIRO, Carolina, VALLS VIDAL, Cristina, VALENZUELA MAYORGA, Susana valeria, RONCERO, M. blanca, PASTOR BLASCO, Francisco i. javier, DÍAZ LUCEA, Pilar, MARTÍNEZ MARTÍNEZ, Josefina. Bacterial cellulose matrices to develop enzymatically active paper. _Cellulose_. 2020. Vol. 27, núm. 3413-3426. [consulta: 20 de gener de 2026]. ISSN: 0969-0239. [Disponible a: https://hdl.handle.net/2445/160957]