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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/62005
Oleoyl Coenzyme A regulates interaction of transcriptional regulator RaaS (Rv1219c) with DNA in Mycobacteria
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We have recently shown that RaaS (regulator of antimicrobial-assisted survival), encoded by Rv1219c in Mycobacterium tuberculosis and by bcg_1279c in Mycobacterium bovis bacillus Calmette-Guérin, plays an important role in mycobacterial survival in prolonged stationary phase and during murine infection. Here, we demonstrate that long chain acyl-CoA derivatives (oleoyl-CoA and, to lesser extent, palmitoyl-CoA) modulate RaaS binding to DNA and expression of the downstream genes that encode ATP-dependent efflux pumps. Moreover, exogenously added oleic acid influences RaaS-mediated mycobacterial improvement of survival and expression of the RaaS regulon. Our data suggest that long chain acyl-CoA derivatives serve as biological indicators of the bacterial metabolic state. Dysregulation of efflux pumps can be used to eliminate non-growing mycobacteria.
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TURAPOV, Obolbek, WADDELL, Simon j., BURKE, Bernard, GLENN, Sarah, SARYBAEVA, Asel a., TUDÓ I VILANOVA, Griselda, LABESSE, Gilles, YOUNG, Danielle i., YOUNG, Michael, ANDREW, Peter w., BUTCHER, Philip d., COHEN GONSAUN, Martin, MUKAMOLOVA, Galina. Oleoyl Coenzyme A regulates interaction of transcriptional regulator RaaS (Rv1219c) with DNA in Mycobacteria. _Journal of Biological Chemistry_. 2014. Vol. 289, núm. 36, pàgs. 25241-25249. [consulta: 20 de desembre de 2025]. ISSN: 0021-9258. [Disponible a: https://hdl.handle.net/2445/62005]