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Article

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Published version

Publication date

2014-05

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Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/59013

Enhancing hepatic fatty acid oxidation as a strategy for reversing metabolic disorders programmed by maternal undernutrition during gestation

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http://dx.doi.org/10.1159/000358714

Abstract

Background/Aims: Moderate maternal calorie-restriction during gestation programmes offspring for a major propensity to develop metabolic alterations in adulthood. We aimed to assess whether increased hepatic fatty-acid oxidation (FAO), at early ages, by gene transfer of Cpt1am (active mutant of carnitine palmitoyltransferase-1a), may be a strategy for reversing metabolic disturbances associated to maternal calorie-restriction during gestation in rats. Methods: AAV-Gfp (control) and AAV-Cpt1am vectors were administered by tail vein injection in 18-day-old control-pups and the offspring of 20% calorie-restricted rats during gestation (CR). After weaning, animals were fed with normal-fat diet. At the age of 4 months, they were moved to HF-diet and sacrificed at the age of 6 months to collect tissues. Locomotive activity, energy expenditure and blood pressure were measured. Results: Under HF-diet, CR-animals showed higher HOMA-IR, adipocyte diameter and hepatic triglyceride accumulation than controls; these alterations were reverted in Cpt1am-injected animals. In liver, this treatment ameliorated inflammatory state, decreased expression of lipogenesis-related genes and partially restored the decreased expression of leptin-receptor occurring in CR-animals. Treatment also reverted the decreased energy expenditure and the increased blood pressure of CR-animals. Conclusion: Increasing hepatic FAO through AAV-Cpt1am injection at juvenile ages prevents some metabolic disorders associated to gestational maternal calorie-restriction.

Subject

Àcids grassos, Dieta hipocalòrica, Fetge, Teràpia genètica, Malnutrició, Complicacions en l'embaràs, Trastorns del metabolisme

Subject (English)

Fatty acids, Low-calorie diet, Liver, Gene therapy, Malnutrition, Disorders of metabolism, Complications of pregnancy

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Articles publicats en revistes (Bioquímica i Biomedicina Molecular)
Articles publicats en revistes (Institut de Biomedicina (IBUB))

Citation

TORRENS, Juana María, et al. Enhancing hepatic fatty acid oxidation as a strategy for reversing metabolic disorders programmed by maternal undernutrition during gestation. Cellular Physiology and Biochemistry. 2014. Vol. 33, num. 5, pags. 1498-1515. ISSN 1015-8987. [consulted: 9 of June of 2026]. Available at: https://hdl.handle.net/2445/59013

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