Fitxers

123030.pdf (5.78 MB)

Tipus de document

Article

Versió

Versió acceptada

Data de publicació

2008-11-28

Tots els drets reservats

Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/225886

Characterization of the gene family encoding acetoacetyl-CoA thiolase in Arabidopsis

Títol de la revista

Autors

Director/Tutor

ISSN de la revista

Títol del volum

Recurs relacionat

https://doi.org/10.1071/FP08012

Resum

Thiolases are ubiquitous enzymes involved in many essential biochemical processes. Biosynthetic thiolases, also known as acetoacetyl-CoA thiolases (AACT), catalyse a reversible Claisen-type condensation of two acetyl-CoA molecules to form acetoacetyl-CoA. Here, we report the characterisation of two genes from Arabidopsis thaliana L., ACT1 and ACT2, which encode two closely related AACT isoforms (AACT1 and AACT2, respectively). Transient expression of constructs encoding AACT1 and AACT2 fused to GFP revealed that the two proteins show a different subcellular localisation. While AACT1 is found in peroxisomes, AACT2 localises in the cytosol and the nucleus. The peroxisomal localisation of AACT1 depends on the presence of a C-terminal peroxisomal targeting sequence (PTS1) motif (Ser-Ala-Leu) not previously found in other organisms. ACT1 and ACT2 genes are also differentially expressed. Whereas ACT2 is expressed at relatively high level in all plant tissues, the expression of ACT1 is restricted to roots and inflorescences and its transcript is present at very low levels. The obtained results are in agreement with the involvement of AACT2 in catalysing the first step of the mevalonate pathway. The metabolic function of AACT1 is not clear at present, although its particular peroxisomal localisation might exclude a role in isoprenoid biosynthesis.

Matèries

Biologia molecular vegetal, Genètica molecular vegetal

Matèries (anglès)

Plant molecular biology, Plant molecular genetics

Citació

Col·leccions

Articles publicats en revistes (Bioquímica i Biomedicina Molecular)

Citació

AHUMADA, Iván, et al. Characterization of the gene family encoding acetoacetyl-CoA thiolase in Arabidopsis. Functional Plant Biology. 2008. Vol. 35, num. 11, pags. 1100-1111. ISSN 1445-4408. [consulted: 23 of May of 2026]. Available at: https://hdl.handle.net/2445/225886

Exportar metadades

JSON - METS

Compartir registre