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2003-04

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cc-by-nc-nd (c) Universidad de Murcia, 2003
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/104340

Effects of the proapoptotic drug prodigiosin on cell cycle-related proteins in Jurkat T cells

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Abstract

Prodigiosin (PG) is a red pigment produced by Serratia marcescens with immunosuppressive and apoptotic activities. In this study, we sought to examine the effect of PG on cell cycle-related proteins. The antiproliferative activity of PG was tested using human Jurkat leukaemia T cells in culture. PG-inhibited cell proliferation was determined using thymidine incorporation assay. PG-arrested cell cycle was analysed using immunoblot analysis with specific antibodies against cell cycle-related proteins and kinase assays of cdk2. Apoptosis was determined by Hoechst staining and analysis of DNA fragmentation. PG inhibited cyclin E, cdk2, p27 and p21, the induction of the cyclin A-cdk2 and cyclin E-cdk2 kinase activity, and the phosphorylation of Rb in leukaemic Jurkat cells. We confirmed that PG induces apoptosis by the characteristic DNA laddering pattern and condensed nuclei or apoptotic bodies identified by fluorescence microscopy. These results indicate that PG and other family members form a new group of molecules with a common mechanism of action and specific molecular targets, raising the possibility of their therapeutic use as antineoplastic drugs.

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Serratia marcescens, Apoptosi, Proliferació cel·lular, Quimioteràpia, Immunosupressió, Cèl·lules T, Cicle cel·lular, Drogues, Proteïnes, Divisió cel·lular

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Serratia marcescens, Apoptosis, Cell proliferation, Chemotherapy, Immunosuppression, T cells, Cell cycle, Drugs of abuse, Proteins, Cell division

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Articles publicats en revistes (Patologia i Terapèutica Experimental)

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PÉREZ TOMÁS, Ricardo E. and MONTANER RAMONEDA, Beatriz. Effects of the proapoptotic drug prodigiosin on cell cycle-related proteins in Jurkat T cells. Histology and Histopathology. 2003. Vol. 18, num. 2, pags. 379-385. ISSN 0213-3911. [consulted: 11 of August of 2026]. Available at: https://hdl.handle.net/2445/104340

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