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665841.pdf (1.25 MB)

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2012-04

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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/107045

Cyclin A1 is essential for setting the pluripotent state and reducing tumorigenicity of induced pluripotent stem cells

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Recurs relacionat

https://doi.org/10.1089/scd.2012.0190

Resum

The proper differentiation and threat of cancer rising from the application of induced pluripotent stem (iPS) cells are major bottlenecks in the field and are thought to be inherently linked to the pluripotent nature of iPS cells. To address this question, we have compared iPS cells to embryonic stem cells (ESCs), the gold standard of ground state pluripotency, in search for proteins that may improve pluripotency of iPS cells. We have found that when reprogramming somatic cells toward pluripotency, 1%-5% of proteins of 5 important cell functions are not set to the correct expression levels compared to ESCs, including mainly cell cycle proteins. We have shown that resetting cyclin A1 protein expression of early-passage iPS cells closer to the ground state pluripotent state of mouse ESCs improves the pluripotency and reduces the threat of cancer of iPS cells. This work is a proof of principle that reveals that setting expression of certain proteins correctly during reprogramming is essential for achieving ESC-state pluripotency. This finding would be of immediate help to those researchers in different fields of iPS cell work that specializes in cell cycle, apoptosis, cell adhesion, cell signaling, and cytoskeleton.

Matèries

Cèl·lules mare, Cèl·lules mare embrionàries, Cicle cel·lular, Transformació cel·lular, Proteïnes supressores de tumors

Matèries (anglès)

Stem cells, Embryonic stem cells, Cell cycle, Cell transformation, Tumor suppressor protein

Citació

Col·leccions

Articles publicats en revistes (Patologia i Terapèutica Experimental)
Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))
Articles publicats en revistes (Institut de Biomedicina (IBUB))

Citació

MCLENACHAN, Samuel, MENCHÓN NAJAS, Cristina, RAYA CHAMORRO, Ángel, CONSIGLIO, Antonella, EDEL, Michael john. Cyclin A1 is essential for setting the pluripotent state and reducing tumorigenicity of induced pluripotent stem cells. _Stem Cells and Development_. 2012. Vol. 21, núm. 15, pàgs. 2891-2899. [consulta: 2 de gener de 2026]. ISSN: 1547-3287. [Disponible a: https://hdl.handle.net/2445/107045]

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