Characterization of two membrane-associated β-glucosidases from maize coleoptiles

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dc.contributor.authorFeldwisch, Joachim
dc.contributor.authorVente, Andreas
dc.contributor.authorZettl, Rolf
dc.contributor.authorBako, Laszlo
dc.contributor.authorCampos Martínez, Narciso
dc.contributor.authorPalme, Klaus
dc.date.accessioned2026-01-22T11:48:01Z
dc.date.available2026-01-22T11:48:01Z
dc.date.issued1994-08-15
dc.date.updated2026-01-22T11:48:01Z
dc.description.abstractWe isolated membrane vesicles from maize (Zea mays L.) coleoptiles and identified in these vesicles a 58 kDa (pm58) and a 60 kDa (pm60) protein by photoaffinity labelling with 5-azido-[7-3H]indole-3-acetic acid ([3H]N3IAA). Photoaffinity labelling was effectively competed for by auxins as well as by flavonoids. The labelled proteins were solubilized by Triton X-114 from the vesicles and partially purified. Microsequence analysis revealed that pm60 is a beta-glucosidase. This was confirmed by biochemical and immunological analysis. We show that pm60 has a beta-D-glucoside glucohydrolase (EC 3.2.1.21) activity. It uses p-nitro-phenyl beta-D-glucopyranoside (PNPG) as a substrate, with a pH optimum of 5.0. The Km for PNPG is 0.652 mM and the Vmax. 6.24 mumol.min-1.mg-1. The beta-glucosidase activity of pm60 was competitively inhibited by IAA and 1-naphthylacetic acid as well as by gluconolactam and glucose. N-terminal amino-acid-sequence analysis of pm58 revealed similarity to pm60, suggesting that both proteins are encoded by different members of a gene family.
dc.format.extent7 p.
dc.format.mimetypeapplication/pdf
dc.identifier.idgrec086860
dc.identifier.issn0264-6021
dc.identifier.urihttps://hdl.handle.net/2445/225948
dc.language.isoeng
dc.publisherBiochemical Society
dc.relation.isformatofVersió postprint del document publicat a: https://doi.org/10.1042/bj3020015
dc.relation.ispartofBiochemical Journal, 1994, vol. 302, num.1, p. 15-21
dc.relation.urihttps://doi.org/10.1042/bj3020015
dc.rights(c) Feldwisch, J. et al., 1994
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.sourceArticles publicats en revistes (Bioquímica i Biomedicina Molecular)
dc.subject.classificationGlucòsids
dc.subject.classificationBiologia molecular vegetal
dc.subject.otherGlucosides
dc.subject.otherPlant molecular biology
dc.titleCharacterization of two membrane-associated β-glucosidases from maize coleoptiles
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/acceptedVersion

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