Miniatura

Fitxers

714994.pdf (9.32 MB)

Tipus de document

Article

Versió

Versió publicada

Data de publicació

2021-08-24

Llicència de publicació

cc-by (c) Grados Torrez, Ricardo Enrique et al., 2021
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/180688

Loose Morphology and High Dynamism of OSER Structures Induced by the Membrane Domain of HMG-CoA Reductase

Títol de la revista

Autors

Director/Tutor

ISSN de la revista

Títol del volum

Recurs relacionat

https://doi.org/10.3390/ijms22179132

Resum

The membrane domain of eukaryotic HMG-CoA reductase (HMGR) has the conserved capacity to induce endoplasmic reticulum (ER) proliferation and membrane association into Organized Smooth Endoplasmic Reticulum (OSER) structures. These formations develop in response to overexpression of particular proteins, but also occur naturally in cells of the three eukaryotic kingdoms. Here, we characterize OSER structures induced by the membrane domain of Arabidopsis HMGR (1S domain). Immunochemical confocal and electron microscopy studies demonstrate that the 1S:GFP chimera co-localizes with high levels of endogenous HMGR in several ER compartments, such as the ER network, the nuclear envelope, the outer and internal membranes of HMGR vesicles and the OSER structures, which we name ER-HMGR domains. After highpressure freezing, ER-HMGR domains show typical crystalloid, whorled and lamellar ultrastructural patterns, but with wide heterogeneous luminal spaces, indicating that the native OSER is looser and more flexible than previously reported. The formation of ER-HMGR domains is reversible. OSER structures grow by incorporation of ER membranes on their periphery and progressive compaction to the inside. The ER-HMGR domains are highly dynamic in their formation versus their disassembly, their variable spherical-ovoid shape, their fluctuating borders and their rapid intracellular movement, indicating that they are not mere ER membrane aggregates, but active components of the eukaryotic cell.

Matèries

Reticle endoplasmàtic, Refrigeració, Àcid mevalònic

Matèries (anglès)

Endoplasmic reticulum, Cooling, Mevalonic acid

Citació

Col·leccions

Articles publicats en revistes (Bioquímica i Biomedicina Molecular)

Citació

GRADOS TORREZ, Ricardo enrique, LÓPEZ-IGLESIAS, Carmen, FERRER ARTIGAS, Joan carles, CAMPOS MARTÍNEZ, Narciso. Loose Morphology and High Dynamism of OSER Structures Induced by the Membrane Domain of HMG-CoA Reductase. _International Journal of Molecular Sciences_. 2021. Vol. 22, núm. 17, pàgs. 9132. [consulta: 25 de gener de 2026]. ISSN: 1661-6596. [Disponible a: https://hdl.handle.net/2445/180688]

Exportar metadades

JSON - METS

Compartir registre