Fitxers

64515.pdf (574.01 KB)

Tipus de document

Article

Versió

Versió acceptada

Data de publicació

2000-05-19

Tots els drets reservats

Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/225937

Genetic evidence of branching in the isoprenoid pathway for the production of isopentenyl diphosphate and dimethylallyl diphosphate in Escherichia coli

Títol de la revista

Autors

Director/Tutor

ISSN de la revista

Títol del volum

Recurs relacionat

https://doi.org/10.1016/S0014-5793(00)01552-0

Resum

An alternative mevalonate-independent pathway for isoprenoid biosynthesis has been recently discovered in eubacteria (including Escherichia coli) and plant plastids, although it is not fully elucidated yet. In this work, E. coli cells were engineered to utilize exogenously provided mevalonate and used to demonstrate by a genetic approach that branching of the endogenous pathway results in separate synthesis of the isoprenoid building units isopentenyl diphosphate (IPP) and its isomer dimethylallyl diphosphate (DMAPP). In addition, the IPP isomerase encoded by the idi gene was shown to be functional in vivo and to represent the only possibility for interconverting IPP and DMAPP in this bacterium.

Matèries

Escheríchia coli, Microbiologia molecular

Matèries (anglès)

Escherichia coli, Molecular microbiology

Citació

Col·leccions

Articles publicats en revistes (Bioquímica i Biomedicina Molecular)

Citació

RODRÍGUEZ CONCEPCIÓN, Manuel, et al. Genetic evidence of branching in the isoprenoid pathway for the production of isopentenyl diphosphate and dimethylallyl diphosphate in Escherichia coli. FEBS Letters. 2000. Vol. 473, num. 3, pags. 328-332. ISSN 0014-5793. [consulted: 25 of May of 2026]. Available at: https://hdl.handle.net/2445/225937

Exportar metadades

JSON - METS

Compartir registre