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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/112611
The incC sequence is required for R27 plasmid stability
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IncHI plasmids account for multiple antimicrobial resistance in Salmonella and other enterobacterial genera. These plasmids are generally very stable in their bacterial hosts. R27 is the archetype of IncHI1 plasmids. A high percentage of the R27-encoded open reading frames (ORFs) (66.7%) do not show similarity to any known ORFs. We performed a deletion analysis of all non-essential R27 DNA sequences to search for hitherto non-identified plasmid functions that might be required for plasmid stability. We report the identification of a short DNA sequence (incC) that is essential for R27 stability. That region contains several repeats (incC repeats), belongs to one of the three-plasmid replicons (R27 FIA-like) and is targeted by the R27 E protein. Deletion of the incC sequence drastically reduces R27 stability both in Escherichia coli and in Salmonella, the effect being more pronounced in this latter species. Interfering with incC-E protein interaction must lead to a reduced IncHI1 plasmid stability, and may represent a new approach to combat antimicrobial resistance.
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TASSINARI, Eleonora, AZNAR VICENTE, Sonia, URCOLA, Imanol, PRIETO DURÁN, Alejandro, HÜTTENER QUEIROZ, Mário, JUÁREZ GIMÉNEZ, Antonio. The incC sequence is required for R27 plasmid stability. _Frontiers in Microbiology_. 2016. Vol. 7, núm. 6, pàgs. 629. [consulta: 23 de gener de 2026]. ISSN: 1664-302X. [Disponible a: https://hdl.handle.net/2445/112611]