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AdvMatTec_2022_CluaRamon_CollagenTannic.pdf (3.19 MB)

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2022-02-20

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cc by-nc (c) Clua Ferré, Laura et al., 2022
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/183436

Collagen-Tannic Acid Spheroids for β-Cell Encapsulation Fabricated Using a 3D Bioprinter

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https://doi.org/10.1002/admt.202101696

Resum

Type 1 Diabetes results from autoimmune response elicited against β-cell antigens. Nowadays, insulin injections remain the leading therapeutic option. However, injection treatment fails to emulate the highly dynamic insulin release that β-cells provide. 3D cell-laden microspheres have been proposed during the last years as a major platform for bioengineering insulin-secreting constructs for tissue graft implantation and a model for in vitro drug screening platforms. Current microsphere fabrication technologies have several drawbacks: the need for an oil phase containing surfactants, diameter inconsistency of the microspheres, and high time-consuming processes. These technologies have widely used alginate for its rapid gelation, high processability, and low cost. However, its low biocompatible properties do not provide effective cell attachment. This study proposes a high-throughput methodology using a 3D bioprinter that employs an ECM-like microenvironment for effective cell-laden microsphere production to overcome these limitations. Crosslinking the resulting microspheres with tannic acid prevents collagenase degradation and enhances spherical structural consistency while allowing the diffusion of nutrients and oxygen. The approach allows customization of microsphere diameter with extremely low variability. In conclusion, a novel bio-printing procedure is developed to fabricate large amounts of reproducible microspheres capable of secreting insulin in response to extracellular glucose stimuli.

Matèries

Enginyeria biomèdica, Diabetis, Materials biomèdics

Matèries (anglès)

Biomedical engineering, Diabetes, Biomedical materials

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Col·leccions

Articles publicats en revistes (Enginyeria Electrònica i Biomèdica)
<b>Publicacions de projectes de recerca finançats per la UE</b>
Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)
Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))

Citació

CLUA FERRÉ, Laura, DE CHIARA, Francesco, RODRÍGUEZ COMAS, Júlia, COMELLES PUJADAS, Jordi, MARTÍNEZ FRAIZ, Elena, GODEAU, Amelie luise, GARCÍA ALAMÁN, Ainhoa, GASA, Rosa, RAMÓN AZCÓN, Javier. Collagen-Tannic Acid Spheroids for β-Cell Encapsulation Fabricated Using a 3D Bioprinter. _Advanced Materials Technologies_. 2022. Vol. 2101696. [consulta: 25 de febrer de 2026]. ISSN: 2365-709X. [Disponible a: https://hdl.handle.net/2445/183436]

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