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AdvMatTec_2022_CluaRamon_CollagenTannic.pdf (3.19 MB)

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2022-02-20

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cc by-nc (c) Clua Ferré, Laura et al., 2022
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/183436

Collagen-Tannic Acid Spheroids for β-Cell Encapsulation Fabricated Using a 3D Bioprinter

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https://doi.org/10.1002/admt.202101696

Abstract

Type 1 Diabetes results from autoimmune response elicited against β-cell antigens. Nowadays, insulin injections remain the leading therapeutic option. However, injection treatment fails to emulate the highly dynamic insulin release that β-cells provide. 3D cell-laden microspheres have been proposed during the last years as a major platform for bioengineering insulin-secreting constructs for tissue graft implantation and a model for in vitro drug screening platforms. Current microsphere fabrication technologies have several drawbacks: the need for an oil phase containing surfactants, diameter inconsistency of the microspheres, and high time-consuming processes. These technologies have widely used alginate for its rapid gelation, high processability, and low cost. However, its low biocompatible properties do not provide effective cell attachment. This study proposes a high-throughput methodology using a 3D bioprinter that employs an ECM-like microenvironment for effective cell-laden microsphere production to overcome these limitations. Crosslinking the resulting microspheres with tannic acid prevents collagenase degradation and enhances spherical structural consistency while allowing the diffusion of nutrients and oxygen. The approach allows customization of microsphere diameter with extremely low variability. In conclusion, a novel bio-printing procedure is developed to fabricate large amounts of reproducible microspheres capable of secreting insulin in response to extracellular glucose stimuli.

Subject

Enginyeria biomèdica, Diabetis, Materials biomèdics

Subject (English)

Biomedical engineering, Diabetes, Biomedical materials

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Collections

Articles publicats en revistes (Enginyeria Electrònica i Biomèdica)
Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)
Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))
Publicacions de projectes de recerca finançats per la UE

Citation

CLUA FERRÉ, Laura, et al. Collagen-Tannic Acid Spheroids for β-Cell Encapsulation Fabricated Using a 3D Bioprinter. Advanced Materials Technologies. 2022. Vol. 2101696. ISSN 2365-709X. [consulted: 11 of August of 2026]. Available at: https://hdl.handle.net/2445/183436

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