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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/179528
Adipose tissue glycogen accumulation is associated with obesity-linked inflammation in humans
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Objective: glycogen metabolism has emerged as a mediator in the control of energy homeostasis and studies in murine models reveal that adipose tissue might contain glycogen stores. Here we investigated the physio(patho)logical role of glycogen in human adipose tissue in the context of obesity and insulin resistance. Methods: we studied glucose metabolic flux of hypoxic human adipoctyes by nuclear magnetic resonance and mass spectrometry-based metabolic approaches. Glycogen synthesis and glycogen content in response to hypoxia was analyzed in human adipocytes and macrophages. To explore the metabolic effects of enforced glycogen deposition in adipocytes and macrophages, we overexpressed PTG, the only glycogen-associated regulatory subunit (PP1-GTS) reported in murine adipocytes. Adipose tissue gene expression analysis was performed on wild type and homozygous PTG KO male mice. Finally, glycogen metabolism gene expression and glycogen accumulation was analyzed in adipose tissue, mature adipocytes and resident macrophages from lean and obese subjects with different degrees of insulin resistance in 2 independent cohorts. Results: we show that hypoxia modulates glucose metabolic flux in human adipocytes and macrophages and promotes glycogenesis. Enforced glycogen deposition by overexpression of PTG re-orients adipocyte secretion to a pro-inflammatory response linked to insulin resistance and monocyte/lymphocyte migration. Furthermore, glycogen accumulation is associated with inhibition of mTORC1 signaling and increased basal autophagy flux, correlating with greater leptin release in glycogen-loaded adipocytes. PTG-KO mice have reduced expression of key inflammatory genes in adipose tissue and PTG overexpression in M0 macrophages induces a pro-inflammatory and glycolytic M1 phenotype. Increased glycogen synthase expression correlates with glycogen deposition in subcutaneous adipose tissue of obese patients. Glycogen content in subcutaneous mature adipocytes is associated with BMI and leptin expression. Conclusion: our data establish glycogen mishandling in adipose tissue as a potential key feature of inflammatory-related metabolic stress in human obesity.
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CEPERUELO-MALLAFRÉ, Victoria, EJARQUE, Miriam, SERENA, Carolina, DURAN, Xavier, MONTORI GRAU, Marta, RODRÍGUEZ, Miguel angel, YANES, Oscar, NÚÑEZ-ROA, Catalina, ROCHE, Kelly, PUTHANVEETIL, Prasanth, GARRIDO-SANCHEZ, Lourdes, SAEZ, Enrique, TINAHONES, Francisco j., GARCÍA-ROVES, Pablo m. (pablo miguel), GÓMEZ FOIX, Anna maria, SALTIEL, Alan r., VENDRELL, Joan, FERNÁNDEZ-VELEDO, Sonia. Adipose tissue glycogen accumulation is associated with obesity-linked inflammation in humans. _Molecular Metabolism_. 2015. Vol. 5, núm. 1, pàgs. 5-18. [consulta: 21 de gener de 2026]. ISSN: 2212-8778. [Disponible a: https://hdl.handle.net/2445/179528]