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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/171714
Differential regulation of CsrC and CsrB by CRP-cAMP in Salmonella enterica
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Post-transcriptional regulation mediated by regulatory small RNAs (sRNAs) has risen as a key player in fine-tuning gene expression in response to environmental stimuli. Here, we show that, in Salmonella enterica, the central metabolic regulator CRP-cAMP differentially regulates the sRNAs CsrB and CsrC in a growth phase-dependent manner. While CsrB expression remains unchanged during growth, CsrC displays a growth phase-dependent expression profile, being weakly expressed at the logarithmic growth phase and induced upon entry into stationary phase. We show that CRP-cAMP contributes to the expression pattern of CsrC by repressing its expression during the logarithmic growth phase. The CRP-cAMP mediated repression of CsrC is independent of SirA, a known transcriptional CsrB/CsrC activator. We further show that the sRNA Spot 42, which is derepressed in a Δcrp strain, upregulates CsrC during logarithmic growth. We propose a model where the growth-dependent regulation of CsrC is sustained by the CRP-cAMP-mediated repression of Spot 42. Together, our data point toward a differential regulation of the sRNAs CsrB and CsrC in response to environmental stimuli, leading to fine-tuning of gene expression via the sequestration of the RNA-binding protein CsrA.
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EL MOUALI BENOMAR, Youssef, ESTEVA-MARTÍNEZ, Guillem, GARCÍA-PEDEMONTE, David, BALSALOBRE PARRA, Carlos. Differential regulation of CsrC and CsrB by CRP-cAMP in Salmonella enterica. _Frontiers in Microbiology_. 2020. Vol. 11, núm. 570536. [consulta: 25 de febrer de 2026]. ISSN: 1664-302X. [Disponible a: https://hdl.handle.net/2445/171714]