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2020-10-14

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cc-by (c) El Mouali, Youssef et al., 2020
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/171714

Differential regulation of CsrC and CsrB by CRP-cAMP in Salmonella enterica

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Recurs relacionat

https://doi.org/10.3389/fmicb.2020.570536

Resum

Post-transcriptional regulation mediated by regulatory small RNAs (sRNAs) has risen as a key player in fine-tuning gene expression in response to environmental stimuli. Here, we show that, in Salmonella enterica, the central metabolic regulator CRP-cAMP differentially regulates the sRNAs CsrB and CsrC in a growth phase-dependent manner. While CsrB expression remains unchanged during growth, CsrC displays a growth phase-dependent expression profile, being weakly expressed at the logarithmic growth phase and induced upon entry into stationary phase. We show that CRP-cAMP contributes to the expression pattern of CsrC by repressing its expression during the logarithmic growth phase. The CRP-cAMP mediated repression of CsrC is independent of SirA, a known transcriptional CsrB/CsrC activator. We further show that the sRNA Spot 42, which is derepressed in a Δcrp strain, upregulates CsrC during logarithmic growth. We propose a model where the growth-dependent regulation of CsrC is sustained by the CRP-cAMP-mediated repression of Spot 42. Together, our data point toward a differential regulation of the sRNAs CsrB and CsrC in response to environmental stimuli, leading to fine-tuning of gene expression via the sequestration of the RNA-binding protein CsrA.

Matèries

Salmonel·la, Expressió gènica

Matèries (anglès)

Salmonella, Gene expression

Citació

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Articles publicats en revistes (Genètica, Microbiologia i Estadística)

Citació

EL MOUALI BENOMAR, Youssef, et al. Differential regulation of CsrC and CsrB by CRP-cAMP in Salmonella enterica. Frontiers in Microbiology. 2020. Vol. 11, num. 570536. ISSN 1664-302X. [consulted: 18 of August of 2026]. Available at: https://hdl.handle.net/2445/171714

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