Amb motiu del tancament d'estiu, la validació de documents es reprendrà a partir del 28 d'agost de 2026. Disculpeu les molèsties.
Con motivo del cierre de verano, la validación de documentos se reanudará a partir del 28 de agosto de 2026. Disculpad las molestias
Due to the summer closure, document validation will resume starting August 28, 2026. We apologize for any inconvenience.

Files

639334.pdf (707.03 KB)

Document type

Article

Version

Published version

Publication date

2013-12-20

Publication license

cc-by (c) Madeira, Ana et al., 2013
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/124629

Biophysical assessment of human aquaporin-7 as a water and glycerol channel in 3T3-L1 adipocytes

Journal Title

Authors

Director/Tutor

Journal ISSN

Volume Title

Related resource

https://doi.org/10.1371/journal.pone.0083442

Abstract

The plasma membrane aquaporin-7 (AQP7) has been shown to be expressed in adipose tissue and its role in glycerol release/uptake in adipocytes has been postulated and correlated with obesity onset. However, some studies have contradicted this view. Based on this situation, we have re-assessed the precise localization of AQP7 in adipose tissue and analyzed its function as a water and/or glycerol channel in adipose cells. Fractionation of mice adipose tissue revealed that AQP7 is located in both adipose and stromal vascular fractions. Moreover, AQP7 was the only aquaglyceroporin expressed in adipose tissue and in 3T3-L1 adipocytes. By overexpressing the human AQP7 in 3T3-L1 adipocytes it was possible to ascertain its role as a water and glycerol channel in a gain-of-function scenario. AQP7 expression had no effect in equilibrium cell volume but AQP7 loss of function correlated with higher triglyceride content. Furthermore it is also reported for the first time a negative correlation between water permeability and the cell non-osmotic volume supporting the observation that AQP7 depleted cells are more prone to lipid accumulation. Additionally, the strong positive correlation between the rates of water and glycerol transport highlights the role of AQP7 as both a water and a glycerol channel and reflects its expression levels in cells. In all, our results clearly document a direct involvement of AQP7 in water and glycerol transport, as well as in triglyceride content in adipocytes.

Subject

Cèl·lules animals, Glicerina, Teixit adipós

Subject (English)

Animal cells, Glycerin, Adipose tissues

Citation

Collections

Articles publicats en revistes (Bioquímica i Biomedicina Molecular)

Citation

MADEIRA, Ana, et al. Biophysical assessment of human aquaporin-7 as a water and glycerol channel in 3T3-L1 adipocytes. PLoS One. 2013. Vol. 8, num. 12, pags. 1-10. ISSN 1932-6203. [consulted: 9 of August of 2026]. Available at: https://hdl.handle.net/2445/124629

Export metadata

JSON - METS

Share record