Amb motiu del tancament d'estiu, la validació de documents es reprendrà a partir del 28 d'agost de 2026. Disculpeu les molèsties.
Con motivo del cierre de verano, la validación de documentos se reanudará a partir del 28 de agosto de 2026. Disculpad las molestias
Due to the summer closure, document validation will resume starting August 28, 2026. We apologize for any inconvenience.

Files

731921.pdf (1.67 MB)

Document type

Article

Version

Published version

Publication date

2022-09-29

Publication license

cc by (c) López Chicón, Patrícia et., 2022
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/194588

A comparative study using fluorescent confocal microscopy and flow cytometry to evaluate chondrocyte viability in human osteochondral allografts.

Journal Title

Authors

Director/Tutor

Journal ISSN

Volume Title

Related resource

https://doi.org/10.3390/bioengineering9100515

Abstract

The preservation conditions of fresh osteochondral allografts for clinical applications are critical due their objective: to transplant mature hyaline cartilage containing viable chondrocytes, maintaining their metabolic activity and also preserving the structural and functional characteristics of the extracellular matrix. The aim of the present study was to compare fluorescence confocal microscopy and flow cytometry techniques to evaluate the viability of the chondrocytes present in the osteochondral tissue, in order to determine their effectiveness and thus ensure reproducibility and robustness of the analysis. To this end, osteochondral allografts from human cadaveric donors were preserved at 4 °C for 3 weeks in a preservation medium supplemented with antibiotic and antifungal agents. Cell viability of chondrocytes was determined by monitoring the cartilage for 3 weeks of preservation by confocal fluorescence microscopy and flow cytometry, obtaining cell viabilities of 83.7 ± 2.6% and 55.8 ± 7.8% for week three, respectively. The confocal fluorescence microscopy approach is more advantageous and accurate, as it correlates better with actual cell viability values for monitoring osteochondral graft preservation, detecting only the cells that died a natural death associated with the preservation method.

Subject

Microscòpia confocal, Microscòpia de fluorescència, Citometria de fluxe, Rebuig (Biologia), Artrosi

Subject (English)

Confocal microscopy, Fluorescence microscopy, Flow cytometry, Graft rejection, Osteoarthritis

Citation

Collections

Articles publicats en revistes (Cirurgia i Especialitats Medicoquirúrgiques)

Citation

LÓPEZ CHICÓN, Patrícia, et al. A comparative study using fluorescent confocal microscopy and flow cytometry to evaluate chondrocyte viability in human osteochondral allografts. Bioengineering-Basel. 2022. Vol. 9, num. 10, pags. 515. ISSN 2306-5354. [consulted: 15 of August of 2026]. Available at: https://hdl.handle.net/2445/194588

Export metadata

JSON - METS

Share record