Carregant...
Fitxers
Tipus de document
ArticleVersió
Versió publicadaData de publicació
Llicència de publicació
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/194588
A comparative study using fluorescent confocal microscopy and flow cytometry to evaluate chondrocyte viability in human osteochondral allografts.
Títol de la revista
Director/Tutor
ISSN de la revista
Títol del volum
Recurs relacionat
Resum
The preservation conditions of fresh osteochondral allografts for clinical applications are critical due their objective: to transplant mature hyaline cartilage containing viable chondrocytes, maintaining their metabolic activity and also preserving the structural and functional characteristics of the extracellular matrix. The aim of the present study was to compare fluorescence confocal microscopy and flow cytometry techniques to evaluate the viability of the chondrocytes present in the osteochondral tissue, in order to determine their effectiveness and thus ensure reproducibility and robustness of the analysis. To this end, osteochondral allografts from human cadaveric donors were preserved at 4 °C for 3 weeks in a preservation medium supplemented with antibiotic and antifungal agents. Cell viability of chondrocytes was determined by monitoring the cartilage for 3 weeks of preservation by confocal fluorescence microscopy and flow cytometry, obtaining cell viabilities of 83.7 ± 2.6% and 55.8 ± 7.8% for week three, respectively. The confocal fluorescence microscopy approach is more advantageous and accurate, as it correlates better with actual cell viability values for monitoring osteochondral graft preservation, detecting only the cells that died a natural death associated with the preservation method.
Matèries (anglès)
Citació
Citació
LÓPEZ CHICÓN, Patrícia, RIBA TIETZ, Tatiana, FARIÑAS, Óscar, GELBER, Pablo eduardo, CASAROLI MARANO, Ricardo pedro, VILARRODONA, Anna. A comparative study using fluorescent confocal microscopy and flow cytometry to evaluate chondrocyte viability in human osteochondral allografts.. _Bioengineering-Basel_. 2022. Vol. 9, núm. 10, pàgs. 515. [consulta: 15 de gener de 2026]. ISSN: 2306-5354. [Disponible a: https://hdl.handle.net/2445/194588]