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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/175547
Androgen receptor and its splicing variant 7 expression in peripheral blood mononuclear cells and in circulating tumor cells in metastatic castration-resistant prostate cancer
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Androgen receptor (AR) signaling remains crucial in castration-resistant prostate cancer (CRPC). Since it is also essential in immune cells, we studied whether the expression of AR full-length (ARFL) and its splicing variant ARV7 in peripheral blood mononuclear cells (PBMC) predicts systemic treatment response in mCRPC in comparison with circulating-tumor cells (CTC). We measured ARFL and ARV7 mRNA in PBMC and CTC from patients prior to receiving abiraterone (AA), enzalutamide (E), or taxanes by a pre-amplification plus quantitative reverse-transcription PCR. They were also tested in LNCaP-ARV7-transfected and in 22RV1 docetaxel-resistant (22RV1DR) cells. We studied 171 PBMC from 136 patients and from 24 non-cancer controls, and 47 CTC from 22 patients. High PBMC ARV7 levels correlated with worse AA/E and better taxane response. In taxane-treated patients high PBMC ARFL also correlated with longer progression-free survival (PFS). High ARV7 and ARFL expression were independently associated with better biochemical-PFS. Conversely, high CTC ARV7 and ARFL correlated with shorter radiological-PFS and overall survival, respectively. High ARV7 in 22RV1DR and LNCaP-ARV7 cells correlated with taxane resistance. In conclusion, ARFL and ARV7 at PBMC or CTC have a different predictive role in the taxane response, suggesting a potential influence of the AR pathway from PBMC in such response modulation.
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MARÍN AGUILERA, Mercedes, JIMÉNEZ, Natalia, REIG TORRAS, Oscar, MONTALBO CALAFELL, Ruth, VERMA, Ajit k., CASTELLANO, Giancarlo, MENGUAL BRICHS, Lourdes, VICTORIA, Iván, PEREIRA, María verónica, MILÀ GUASCH, Maria, GARCÍA-RECIO, Susana, BENÍTEZ-RIBAS, Daniel, CABEZÓN, Raquel, GONZÁLEZ, Azucena, JUAN, Manel, PRAT APARICIO, Aleix, MELLADO GONZÁLEZ, Begoña. Androgen receptor and its splicing variant 7 expression in peripheral blood mononuclear cells and in circulating tumor cells in metastatic castration-resistant prostate cancer. _Cells_. 2020. Vol. 9, núm. 1, pàgs. 203. [consulta: 23 de gener de 2026]. ISSN: 2073-4409. [Disponible a: https://hdl.handle.net/2445/175547]