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2018-04-30

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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/123707

Use of 14C-glucose by primary cultures of mature rat epididymal adipocytes. Marked release of lactate and glycerol, but limited lipogenesis in the absence of external stimuli

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https://doi.org/10.1080/21623945.2018.1460020

Resum

White adipose tissue can metabolize large amounts of glucose to glycerol and lactate. We quantitatively traced glucose label to lactate, glycerol and fats in primary cultures of mature rat epididymal adipocytes. Cells were incubated with 7/14 mM 14C-glucose for 24/48 h. Medium metabolites and the label in them and in cells' components were measured. Gene expression analysis was done using parallel incubations. Glucose concentration did not affect lactate efflux and most parameters. Glycerol efflux increased after 24h, coinciding with arrested lipogenesis. Steady production of lactate was maintained in parallel to glycerogenesis. Changes in adipocyte metabolism were paralleled by gene expression. Glucose use for lipogenesis was minimal, and stopped (24h-onwards) when glycerol efflux increased because of triacylglycerol turnover. Lactate steady efflux showed that anaerobic glycolysis was the main adipocyte source of energy. We can assume that adipose tissue may play a quantitatively significant effect on glycaemia, returning 3C fragments thus minimizing lipogenesis.

Matèries

Teixit adipós, Metabolisme dels lípids, Glucosa, Rates (Animals de laboratori)

Matèries (anglès)

Adipose tissues, Lipid metabolism, Glucose, Rats as laboratory animals

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Articles publicats en revistes (Bioquímica i Biomedicina Molecular)

Citació

HO-PALMA, Ana cecilia, ROTONDO, Floriana, ROMERO ROMERO, María del mar, FERNÁNDEZ LÓPEZ, José antonio, REMESAR BETLLOCH, Xavier, ALEMANY, Marià. Use of 14C-glucose by primary cultures of mature rat epididymal adipocytes. Marked release of lactate and glycerol, but limited lipogenesis in the absence of external stimuli. _Adipocyte_. 2018. [consulta: 21 de gener de 2026]. ISSN: 2162-3945. [Disponible a: https://hdl.handle.net/2445/123707]

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