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Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/183017
A novel multi-frequency trans-endothelial electrical resistance (MTEER) sensor array to monitor blood-brain barrier integrity
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Giuseppe, Davide di
Mencattini, Arianna
Samitier i Martí, Josep
Martinelli, Eugenio
Badiola Mateos, Maider
Paoli, Roberto
López Martínez, María José
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Abstract
© 2021 Elsevier B.V. The blood-brain barrier (BBB) is a dynamic cellular barrier that regulates brain nutrient supply, waste efflux, and paracellular diffusion through specialized junctional complexes. Finding a system to mimic and monitor BBB integrity (i.e., to be able to assess the effect of certain compounds on opening or closing the barrier) is of vital importance in several pathologies. This work aims to overcome some limitations of current barrier integrity measuring techniques thanks to a multi-layer microfluidic platform with integrated electrodes and Multi-frequency Trans-Endothelial Electrical Resistance (MTEER) in synergy with machine learning algorithms. MTEER measurements are performed across the barrier in a range of frequencies up to 10 MHz highlighting the presence of information on different frequency ranges. Results show that the proposed platform can detect barrier formation, opening, and regeneration afterwards, correlating with the results obtained from immunostaining of junctional complexes. This model presents novel techniques for a future biological barrier in-vitro studies that could potentially help on elucidating barrier opening or sealing on treatments with different drugs.
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GIUSEPPE, Davide di, et al. A novel multi-frequency trans-endothelial electrical resistance (MTEER) sensor array to monitor blood-brain barrier integrity. Sensors And Actuators B-Chemical. 2021. Vol. vol 334. ISSN 0925-4005. [consulted: 7 of June of 2026]. Available at: https://hdl.handle.net/2445/183017