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Bacterial Cellulose-Chitosan paper with antimicrobial and antioxidant activities

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The production of paper-based bacterial cellulose-chitosan (BC-Ch) nanocomposites was accomplished following two different approaches. In the first, BC paper sheets were produced and then immersed in an aqueous solution of chitosan (BC-ChI); in the second, BC pulp was impregnated with chitosan prior to the production of paper sheets (BC-ChM). BC-Ch nanocomposites were investigated in terms of physical characteristics, antimicrobial and antioxidant properties, and the ability to inhibit the formation of biofilms on their surface. The two types of BC-Ch nanocomposites maintained the hydrophobic character, the air barrier properties, and the high crystallinity of the BC paper. However, BC-ChI showed a surface with a denser fiber network and with smaller pores than those of BC-ChM. Only 5% of the chitosan leached from the BC-Ch nanocomposites after 96 h of incubation in an aqueous medium, indicating that it was well retained by the BC paper matrix. BC-Ch nanocomposites displayed antimicrobial activity, inhibiting growth of and having a killing effect against bacteria Staphylococcus aureus and Pseudomonas aeruginosa and yeast Candida albicans. Moreover, BC-Ch papers showed activity against the formation of a biofilm on their surface. The incorporation of chitosan increased the antioxidant activity of the BC paper. Paper-based BC-Ch nanocomposites combined the physical properties of BC paper and the antimicrobial, antibiofilm, and antioxidant activities of chitosan.

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CABAÑAS ROMERO, Lourdes verónica, VALLS VIDAL, Cristina, VALENZUELA MAYORGA, Susana valeria, RONCERO, M. blanca, PASTOR BLASCO, Francisco i. javier, DÍAZ LUCEA, Pilar, MARTÍNEZ MARTÍNEZ, Josefina. Bacterial Cellulose-Chitosan paper with antimicrobial and antioxidant activities. _Biomacromolecules_. 2020. Vol. 21, núm. 4, pàgs. 1568-1577. [consulta: 23 de gener de 2026]. ISSN: 1525-7797. [Disponible a: https://hdl.handle.net/2445/163727]

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