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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/122783
Theoretical and experimental approaches for the initiation and propagation of activity in spatially embedded neuronal cultures
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[eng] Spatial embedding and inherited metric constraints are a fundamental trait of biological neuronal circuits. However their role in shaping connectivity and dynamics has been often disregarded, with models of neuronal networks paying much more attention to the distribution of connections in the quest for understanding network's behavior. In this thesis we aim at filling this gap by studying the importance of metric features in the complex connectivity- dynamics-noise interplay that shapes spontaneous neuronal activity. This thesis combines experiments in rat dissociated neuronal cultures with theoretical analyses to better comprehend the relevance of spatial embedding. We developed a new theoretical model grounded on Ising Models to assess metric effects in neuronal cultures' behavior, and in the context of percolation approaches. Once metric effects were settled, we illustrated their relevance in shaping spontaneous activity by perturbing the structural connectivity blueprint of neuronal cultures. This was achieved by patterning the substrate where neurons grow, and by using topographical molds that dictated the connectivity of the network. Next, and since the initiation of bursting activity is governed in great manner by a complex amplification mechanism that involves metric correlations and noise, we focused on the metric-driven amplification of spontaneous single-neuron noise to derive an analytical model that predicts the frequency of bursting events in neuronal cultures. We then further investigated in an experimental context the contribution of noise to the observed activity patterns, and by implementing a moderate electrical stimulation protocol that increases the level of activity noise in cultures. Finally, the latter study was completed with experiments regarding the specific role of inhibition in neuronal networks, to provide a wider understanding of the mechanisms that govern the initiation and propagation of activity fronts in cortical cultures.
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HERNÁNDEZ NAVARRO, Lluís. Theoretical and experimental approaches for the initiation and propagation of activity in spatially embedded neuronal cultures. [consulta: 13 de desembre de 2025]. [Disponible a: https://hdl.handle.net/2445/122783]