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2022-05-21

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cc-by (c) Molina Ruíz, Francisco J. et al., 2022
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/195640

Standardization of Cell Culture Conditions and Routine Genomic Screening under a Quality Management System Leads to Reduced Genomic Instability in hPSCs

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https://doi.org/10.3390/cells11131984

Resum

Human pluripotent stem cells (hPSCs) have generated unprecedented interest in the scientific community, given their potential applications in regenerative medicine, disease modeling, toxicology and drug screening. However, hPSCs are prone to acquire genomic alterations in vitro, mainly due to suboptimal culture conditions and inappropriate routines to monitor genome integrity. This poses a challenge to both the safety of clinical applications and the reliability of basic and translational hPSC research. In this study, we aim to investigate if the implementation of a Quality Management System (QMS) such as ISO9001:2015 to ensure reproducible and standardized cell culture conditions and genomic screening strategies can decrease the prevalence of genomic alterations affecting hPSCs used for research applications. To this aim, we performed a retrospective analysis of G-banding karyotype and Comparative Genomic Hybridization array (aCGH) data generated by our group over a 5-year span of different hESC and hiPSC cultures. This work demonstrates that application of a QMS to standardize cell culture conditions and genomic monitoring routines leads to a striking improvement of genomic stability in hPSCs cultured in vitro, as evidenced by a reduced probability of potentially pathogenic chromosomal aberrations and subchromosomal genomic alterations. These results support the need to implement QMS in academic laboratories performing hPSC research.

Matèries

Norma ISO 9001, Teràpia cel·lular, Genòmica, Cèl·lules mare, Cariotips, Cultius cel·lulars humans

Matèries (anglès)

ISO 9001 Standard, Cellular therapy, Genomics, Stem cells, Karyotypes, Human cell culture

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Articles publicats en revistes (Biomedicina)
Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)
Articles publicats en revistes (Institut de Neurociències (UBNeuro))

Citació

MOLINA RUIZ, Francisco jose, INTRONA, Clelia, BOMBAU, Georgina, GALOFRÉ, Mireia, CANALS I COLL, Josep m.. Standardization of Cell Culture Conditions and Routine Genomic Screening under a Quality Management System Leads to Reduced Genomic Instability in hPSCs. _Cells_. 2022. Vol. 11, núm. 13, pàgs. 1984. [consulta: 20 de gener de 2026]. ISSN: 2073-4409. [Disponible a: https://hdl.handle.net/2445/195640]

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