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2020-12-11

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cc-by (c) Bajic, Svetlana Sokovic et al., 2020
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/174040

Proteomic profile of extracellular vesicles released by Lactiplantibacillus plantarum BGAN8 and their internalization by non‑polarized HT29 cell line

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Recurs relacionat

https://doi.org/10.1038/s41598-020-78920-z

Resum

In recent years the role of extracellular vesicles (EVs) of Gram-positive bacteria in host-microbe cross-talk has become increasingly appreciated, although the knowledge of their biogenesis, release and host-uptake is still limited. The aim of this study was to characterize the EVs released by the dairy isolate Lactiplantibacillus plantarum BGAN8 and to gain an insight into the putative mechanism of EVs uptake by intestinal epithelial cells. The cryo-TEM observation undoubtedly demonstrated the release of EVs (20 to 140nm) from the surface of BGAN8, with exopolysaccharides seems to be part of EVs surface. The proteomic analysis revealed that the EVs are enriched in enzymes involved in central metabolic pathways, such as glycolysis, and in membrane components with the most abundant proteins belonging to amino acid/peptide ABC transporters. Putative internalization pathways were evaluated in time-course internalization experiments with non-polarized HT29 cells in the presence of inhibitors of endocytic pathways: chlorpromazine and dynasore (inhibitors of clathrin-mediated endocytosis-CME) and filipin III and nystatin (disrupting lipid rafts). For the first time, our results revealed that the internalization was specifically inhibited by dynasore and chlorpromazine but not by filipin III and nystatin implying that one of the entries of L. plantarum vesicles was through CME pathway.

Matèries

Interacció cel·lular, Probiòtics, Bacteris

Matèries (anglès)

Cell interaction, Probiotics, Bacteria

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Col·leccions

Articles publicats en revistes (Bioquímica i Fisiologia)

Citació

BAJIC, Svetlana Sokovic, et al. Proteomic profile of extracellular vesicles released by Lactiplantibacillus plantarum BGAN8 and their internalization by non‑polarized HT29 cell line. Scientific Reports. 2020. Vol. 10, num. 1, pags. 21829. ISSN 2045-2322. [consulted: 3 of August of 2026]. Available at: https://hdl.handle.net/2445/174040

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