Miniatura

Fitxers

193835.pdf (571.35 KB)

Tipus de document

Article

Versió

Versió publicada

Data de publicació

2002-02-01

Tots els drets reservats

Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/177001

Direct binding of Smad1 and Smad4 to two distinct motifs mediates BMP-specific transcriptional activation of Id1 gene

Títol de la revista

Autors

Director/Tutor

ISSN de la revista

Títol del volum

Recurs relacionat

https://doi.org/10.1074/jbc.M106826200

Resum

Bone morphogenetic proteins (BMPs) are potent inhibitors of myoblast differentiation and inducers of bone formation both in vivo and in vitro. Expression of Id1, a negative regulator of basic helix-loop-helix transcription factors, is up-regulated by BMPs and contributes to the antimyogenic effects of this family of cytokines. In this report, we have identified a specific BMP-2 immediate early response enhancer in the human Id1 gene. Transcriptional activation of the enhancer was increased by overexpression of BMP-responsive Smads, and Smad4 and was completely abrogated in Smad4-deficient cells. Deletion analysis demonstrates that the responsive region is composed of two separate DNA binding elements, a set of overlapping GC boxes, which bind BMP-regulated Smads upon BMP stimulation, and three repeats of CAGAC boxes. Gel shift and oligonucleotide pull-down assays demonstrated that these two types of motifs were capable of binding their corresponding Smads. However, deletion or mutation of either DNA binding element was nonadditive, since disruption of either GC or CAGAC boxes resulted in complete or severe loss of BMP-2 responsiveness. These data suggest the simultaneous requirement of two independent DNA binding elements to allow functional cooperativity of BMP-regulated Smads and Smad4 in BMP-activated gene promoters.

Matèries

Proteïnes, ADN, Factors de transcripció

Matèries (anglès)

Proteins, DNA, Transcription factors

Citació

Col·leccions

Articles publicats en revistes (Ciències Fisiològiques)

Citació

LÓPEZ ROVIRA, Teresa, CHALAUX, Elisabet, MASSAGUÉ I SOLÉ, Joan, ROSA LÓPEZ, José luis, VENTURA PUJOL, Francesc. Direct binding of Smad1 and Smad4 to two distinct motifs mediates BMP-specific transcriptional activation of Id1 gene. _Journal of Biological Chemistry_. 2002. Vol. 277, núm. 5, pàgs. 3176-3185. [consulta: 23 de gener de 2026]. ISSN: 0021-9258. [Disponible a: https://hdl.handle.net/2445/177001]

Exportar metadades

JSON - METS

Compartir registre