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2016-07-07

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cc by (c) Anand et al., 2016
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/101553

A novel Plasmodium falciparum rhoptry associated adhesin mediates erythrocyte invasion through the sialic-acid dependent pathway

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http://dx.doi.org/10.1038/srep29185

Resum

Erythrocyte invasion by Plasmodium falciparum merozoites is central to blood-stage infection and malaria pathogenesis. This intricate process is coordinated by multiple parasite adhesins that bind erythrocyte receptors and mediate invasion through several alternate pathways. P. falciparum expresses 2700 genes during the blood-stages, of which the identity and function of many remains unknown. Here, we have identified and characterized a novel P. falciparum rhoptry associated adhesin (PfRA) that mediates erythrocyte invasion through the sialic-acid dependent pathway. PfRA appears to play a significant functional role as it is conserved across different Plasmodium species. It is localized in the rhoptries and further translocated to the merozoite surface. Both native and recombinant PfRA specifically bound erythrocytes in a sialic-acid dependent, chymotrypsin and trypsin resistant manner, which was abrogated by PfRA antibodies confirming a role in erythrocyte invasion. PfRA antibodies inhibited erythrocyte invasion and in combination with antibodies against other parasite ligands produced an additive inhibitory effect, thus validating its important role in erythrocyte invasion. We have thus identified a novel P. falciparum adhesin that binds with a sialic acid containing erythrocyte receptor. Our observations substantiate the strategy to block P. falciparum erythrocyte invasion by simultaneously targeting multiple conserved merozoite antigens involved in alternate invasion pathways.

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Plasmodium falciparum, Malària

Matèries (anglès)

Plasmodium falciparum, Malaria

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Articles publicats en revistes (ISGlobal)

Citació

ANAND, Gaurav, REDDY, K. sony, PANDEY, Alok kumar, MIAN, Syed yusuf, SINGH, Hina, MITTAL, Shivani arora, AMLABU, Emmanuel, BASSAT ORELLANA, Quique, MAYOR APARICIO, Alfredo gabriel, CHAUHAN, Virander singh, GAUR, Deepak. A novel Plasmodium falciparum rhoptry associated adhesin
                mediates erythrocyte invasion through the sialic-acid dependent
                pathway. _Scientific Reports_. 2016. Vol. 6, núm. 29185, pàgs. 1-17. [consulta: 23 de gener de 2026]. ISSN: 2045-2322. [Disponible a: https://hdl.handle.net/2445/101553]

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