Miniatura

Fitxers

720998.pdf (6.99 MB)

Tipus de document

Article

Versió

Versió publicada

Data de publicació

2021-08-25

Llicència de publicació

cc-by (c) Arts, Janine J.G. et al., 2021
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/183786

Endothelial junctional membrane protrusions serve as hotspots for neutrophil transmigration

Títol de la revista

Autors

Director/Tutor

ISSN de la revista

Títol del volum

Recurs relacionat

https://doi.org/10.7554/eLife.66074

Resum

Upon inflammation, leukocytes rapidly transmigrate across the endothelium to enter the inflamed tissue. Evidence accumulates that leukocytes use preferred exit sites, alhough it is not yet clear how these hotspots in the endothelium are defined and how they are recognized by the leukocyte. Using lattice light sheet microscopy, we discovered that leukocytes prefer endothelial membrane protrusions at cell junctions for transmigration. Phenotypically, these junctional membrane protrusions are present in an asymmetric manner, meaning that one endothelial cell shows the protrusion and the adjacent one does not. Consequently, leukocytes cross the junction by migrating underneath the protruding endothelial cell. These protrusions depend on Rac1 activity and by using a photo-activatable Rac1 probe, we could artificially generate local exit-sites for leukocytes. Overall, we have discovered a new mechanism that uses local induced junctional membrane protrusions to facilitate/steer the leukocyte escape/exit from inflamed vessel walls.

Matèries

Endoteli, Leucòcits, Inflamació, Immunologia

Matèries (anglès)

Endothelium, Leucocytes, Inflammation, Immunology

Citació

Col·leccions

Articles publicats en revistes (Ciències Fisiològiques)

Citació

ARTS, Janine j.g., MAHLANDT, Elike k., GRÖNLOH, Max l.b., SCHIMMEL, Lilian, NOORDSTRA, Ivar, GORDON, Emma, VAN STEEN, Abraham c.i., TOL, Simon, WALZOG, Barbara, VAN RIJSSEL, Jos, NOLTE, Martijn a., POSTMA, Marten, KHUON, Satya, HEDDLESTON, John m., WAIT, Eric, CHEW, Teng leong, WINTER, Mark, MONTAÑEZ, Eloi, GOEDHART, Joachim, VAN BUUL, Jaap d.. Endothelial junctional membrane protrusions serve as hotspots for neutrophil transmigration. _eLife_. 2021. [consulta: 23 de gener de 2026]. ISSN: 2050-084X. [Disponible a: https://hdl.handle.net/2445/183786]

Exportar metadades

JSON - METS

Compartir registre