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global-hyperactivation-of-enhancers.pdf (27.42 MB)

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2020-09-28

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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/171031

Global hyperactivation of enhancers stabilizes human and mouse naïve pluripotency through inhibition of CDK8/19 Mediator kinases

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https://doi.org/10.1038/s41556-020-0573-1

Resum

Pluripotent stem cells (PSCs) transition between cell states in vitro and reflect developmental changes in the early embryo. PSCs can be stabilized in the naïve state by blocking extracellular differentiation stimuli, particularly FGF-MEK signaling. Here, we report that multiple features of the naïve state in human and mouse PSCs can be recapitulated without affecting FGF-MEK-signaling or global DNA methylation. Mechanistically, chemical inhibition of CDK8 and CDK19 kinases removes their ability to repress the Mediator complex at enhancers. Thus CDK8/19 inhibition increases Mediator-driven recruitment of RNA Pol II to promoters and enhancers. This efficiently stabilizes the naïve transcriptional program and confers resistance to enhancer perturbation by BRD4 inhibition. Moreover, naïve pluripotency during embryonic development coincides with a reduction in CDK8/19. We conclude that global hyperactivation of enhancers drives naïve pluripotency, and this can be achieved in vitro by inhibiting CDK8/19 kinase activity. These principles may apply to other contexts of cellular plasticity.

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Cèl·lules mare, Metilació, ADN

Matèries (anglès)

Stem cells, Methylation, DNA

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Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))
Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))

Citació

LYNCH, Cian j., BERNAD, Raquel, MARTÍNEZ VAL, Ana, SHAHBAZI, Marta n., NÓBREGA PEREIRA, Sandrina, CALVO SERRANO, Isabel, BLANCO APARICIO, Carmen, TARANTINO, Carolina, GARRETA, Elena, RICHART GINÉS, Laia, ALCAZAR, Noelia, GRAÑA CASTRO, Osvaldo, GÓMEZ LÓPEZ, Gonzalo, AKSOY, Irène, MUÑOZ-MARTÍN, Maribel, MARTINEZ, Sonia, ORTEGA, Sagrario, PRIETO, Susana, SIMBOECK, Elisabeth, CAMASSES, Alain, STEPHAN-OTTO ATTOLINI, Camille, FERNANDEZ, Agustín f., SIERRA, Marta i., FRAGA, Mario f., PASTOR, Joaquín, FISHER, Daniel, MONTSERRAT, Núria, SAVATIER, Pierre, MUÑOZ, Javier, ZERNICKA-GOETZ, Magdalena, SERRANO MARUGÁN, Manuel. Global hyperactivation of enhancers stabilizes human and mouse naïve pluripotency through inhibition of CDK8/19 Mediator kinases. _Nature Cell Biology_. 2020. Vol. 22, núm. 1223-1238. [consulta: 23 de gener de 2026]. ISSN: 1465-7392. [Disponible a: https://hdl.handle.net/2445/171031]

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