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Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/157818
In-vitro study of hierarchical structures: Anodic oxidation and alkaline treatments onto highly rough titanium cold spray coatings for biomedical applications
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Hierarchical structures were obtained applying two different nanotexturing surface treatments onto highly rough commercial pure titanium coatings by cold spray: (i) anodic oxidation and (ii) alkaline treatments. An extended surface characterization in terms of topography, composition, and wettability has been performed to understand how those parameters affect to cell response. Primary human osteoblasts extracted from knee were seeded onto the as-sprayed titanium surface before and after the nanotexturing treatments. Cell viability was tested by using MTS and LIVE/DEAD assays, as well as osteoblasts differentiation by alkaline phosphatase (ALP) quantification at 3 and 10 days of cell culture. The combination of micro-/nano-roughness results in a significantly increase of cell proliferation, as well as cell differentiation after 10 days of cell culture in comparison with the non-treated coatings.
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MARTÍN VILARDELL, Anna, et al. In-vitro study of hierarchical structures: Anodic oxidation and alkaline treatments onto highly rough titanium cold spray coatings for biomedical applications. Materials Science and Engineering: C. 2018. Vol. 91, num. 589-596. ISSN 0928-4931. [consulted: 6 of June of 2026]. Available at: https://hdl.handle.net/2445/157818