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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/176628
Evaluation of a Loop-Mediated Isothermal Amplification Assay to Detect Carbapenemases Directly from Bronchoalveolar Lavage Fluid Spiked with Acinetobacter spp
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Carbapenem-resistant Acinetobacter spp. mainly Acinetobacter baumannii are frequently causing nosocomial infections with high mortality. In this study, the efficacy of the Eazyplex® SuperBug Complete A system, based on loop-mediated isothermal amplification (LAMP), to detect the presence of carbapenemases in Acinetobacter spp. directly from bronchoalveolar lavage (BAL) samples was assessed. A total of 22 Acinetobacter spp. strains producing OXA-23, OXA-40, OXA-58, NDM, and IMP were selected. Eazyplex SuperBug Complete A kit, used with the Genie II device, is a molecular diagnostics kit that detects a selection of genes that express carbapenemases (bla KPC , bla NDM , bla VIM , bla OXA-48 , bla OXA-23 , bla OXA-40 , and bla OXA-58 ). Negative BAL samples were identified, McFarland solutions were prepared from each of the 22 Acinetobacter strains and serial dilutions in saline solution were made to finally spike BAL samples to a concentration of 102 and 103 CFU/ml. Fifteen concentrations out of the 44 tested out did not provide detection of the carbapenemase-producing gene, all but one being at the lowest concentration tested at 102 CFU/ml; therefore, the limit of sensitivity is 103 CFU/ml. This assay represents the kind of advantages that investing in molecular diagnostics brings to the clinical practice, allowing the identification of carbapenemases in less than 30 min with a sensitivity of 103 CFU/ml.
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MORENO MORALES, Javier, VERGARA GÓMEZ, Andrea, KOSTYANEV, Tomislav, RODRÍGUEZ BAÑO, Jesús, GOOSSENS, Herman, VILA ESTAPÉ, Jordi. Evaluation of a Loop-Mediated Isothermal Amplification Assay to Detect Carbapenemases Directly from Bronchoalveolar Lavage Fluid Spiked with Acinetobacter spp. _Frontiers in Microbiology_. 2021. Vol. 11, núm. 597684. [consulta: 14 de abril de 2026]. ISSN: 1664-302X. [Disponible a: https://hdl.handle.net/2445/176628]