Carregant...
Fitxers
Tipus de document
ArticleVersió
Versió publicadaData de publicació
Llicència de publicació
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/217503
Acute paraoxon-induced neurotoxicity in a mouse survival model: Oxidative stress, dopaminergic system alterations and memory deficits<br />
Títol de la revista
Director/Tutor
ISSN de la revista
Títol del volum
Recurs relacionat
Resum
The secondary neurotoxicity induced by severe organophosphorus (OP) poisoning, including paraoxon (POX), is associated with cognitive impairments in survivors, who, despite receiving appropriate emergency treatments, may still experience lasting neurological deficits. Thus, the present study provides a survival mouse model of acute and severe POX poisoning to examine secondary neurotoxicity. Swiss CD-1 male mice were injected with POX (4 mg/kg, s.c.) followed by atropine (4 mg/kg, i.p.), pralidoxime (2-PAM; Pyridine-2-aldoxime methochloride) (25 mg/kg, i.p., twice, 1 h apart) and diazepam (5 mg/kg, i.p.), resulting in a survival rate >90% and Racine score of 5-6. Our results demonstrated that the model showed increased lipid peroxidation, downregulation of antioxidant enzymes and astrogliosis in the mouse hippocampus (HP) and prefrontal cortex (PFC), brain areas involved in cognitive functions. Moreover, dopamine (DA) levels were reduced in the hp, but increased in the PFC. Furthermore, the survival mouse model of acute POX intoxication did not exhibit phenotypic manifestations of depression, anxiety or motor incoordination. However, our results demonstrated long-term recognition memory impairments, which are in accordance with the molecular and neurochemical effects observed. In conclusion, this mouse model can aid in researching POX exposure's effects on memory and developing potential countermeasures against the secondary neurotoxicity induced by severe OP poisoning.
Matèries
Matèries (anglès)
Citació
Citació
URQUIZU, Edurne, PARATUSIC, Selma, GOYENECHEA, Júlia, GÓMEZ-CANELA, Cristian, FUMÀS, Berta, PUBILL SÁNCHEZ, David, RALDÚA, Demetrio, CAMARASA GARCÍA, Jordi, ESCUBEDO RAFA, Elena, LÓPEZ ARNAU, Raúl. Acute paraoxon-induced neurotoxicity in a mouse survival model: Oxidative stress, dopaminergic system alterations and memory deficits<br />. _International Journal of Molecular Sciences_. 2024. Vol. 25, núm. 22. [consulta: 25 de gener de 2026]. ISSN: 1661-6596. [Disponible a: https://hdl.handle.net/2445/217503]