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2023-04-11

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cc-by (c) López-Aguilar E et al., 2023
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/207316

In Vitro and In Vivo Effects of the Combination of Polypurine Reverse Hoogsteen Hairpins against HER-2 and Trastuzumab in Breast Cancer Cells

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Recurs relacionat

https://doi.org/10.3390/ijms24087073

Resum

Therapeutic oligonucleotides are powerful tools for the inhibition of potential targets involved in cancer. We describe the effect of two Polypurine Reverse Hoogsteen (PPRH) hairpins directed against the ERBB2 gene, which is overexpressed in positive HER-2 breast tumors. The inhibition of their target was analyzed by cell viability and at the mRNA and protein levels. The combination of these specific PPRHs with trastuzumab was also explored in breast cancer cell lines, both in vitro and in vivo. PPRHs designed against two intronic sequences of the ERBB2 gene decreased the viability of SKBR-3 and MDA-MB-453 breast cancer cells. The decrease in cell viability was associated with a reduction in ERBB2 mRNA and protein levels. In combination with trastuzumab, PPRHs showed a synergic effect in vitro and reduced tumor growth in vivo. These results represent the preclinical proof of concept of PPRHs as a therapeutic tool for breast cancer

Matèries

Càncer de mama, Cèl·lules canceroses, Oncogens, Receptors cel·lulars

Matèries (anglès)

Breast cancer, Cancer cells, Oncogenes, Cell receptors

Citació

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Articles publicats en revistes (Bioquímica i Fisiologia)

Citació

LÓPEZ-AGUILAR, Ester, et al. In Vitro and In Vivo Effects of the Combination of Polypurine Reverse Hoogsteen Hairpins against HER-2 and Trastuzumab in Breast Cancer Cells. International Journal of Molecular Sciences. 2023. Vol. 24 (8), num. 1, pags. 7073. ISSN 1661-6596. [consulted: 26 of August of 2026]. Available at: https://hdl.handle.net/2445/207316

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