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2019-03-26

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Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/178888

Parallel Clamps and Polypurine Hairpins (PPRH) for Gene Silencing and Triplex-Affinity Capture: Design, Synthesis, and Use

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https://doi.org/10.1002/cpnc.78

Abstract

Nucleic acid triplexes are formed when a DNA or RNA oligonucleotide binds to a polypurine-polypyrimidine-rich sequence. Triplexes have wide therapeutic applications such as gene silencing or site-specific mutagenesis. In addition, protocols based on triplex-affinity capture have been used for detecting nucleic acids in biosensing platforms. In this article, the design, synthesis, and use of parallel clamps and polypurine-reversed hairpins (PPRH) to bind to target polypyrimidine targets are described. The combination of the polypurine Watson-Crick strand with the triplex-forming strand in a single molecule produces highly stable triplexes allowing targeting of single- and double-stranded nucleic acid sequences. On the other hand, PPRHs are easily prepared and work at nanomolar range, like siRNAs, and at a lower concentration than that needed for antisense ODNs or TFOs. However, the stability of PPRHs is higher than that of siRNAs. In addition, PPRHs circumvent off-target effects and are non-immunogenic.

Subject

ADN, Teràpia genètica, Reparació de l'ADN

Subject (English)

DNA, Gene therapy, DNA repair

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Articles publicats en revistes (Bioquímica i Fisiologia)

Citation

AVIÑÓ ANDRÉS, Anna, et al. Parallel Clamps and Polypurine Hairpins (PPRH) for Gene Silencing and Triplex-Affinity Capture: Design, Synthesis, and Use. Current protocols in nucleic acid chemistry. 2019. Vol. 77, num. 1, pags. e78. ISSN 1934-9289. [consulted: 11 of August of 2026]. Available at: https://hdl.handle.net/2445/178888

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