Collective phenomena in neuronal cultures recorded with MEAs

Abstract

This study delves into the dynamics of living neuronal networks using a microelectrode arrays (MEAs) system. Through analysis of the neuronal activity of an in vitro neuronal network, we explored the short– and long–term effects of electrical stimulation on the network’s functional connectivity, which was quantified through cross-correlation in combination with analyses from graph theory. Spatial coarse–sampling of the data revealed a complex relationship between electrode density and network properties, emphasizing the need to examine with care contradictory results in studies with MEAs. Our findings show the intricate interplay between stimulation, plasticity and resilience to damage in neuronal cultures