Carregant...
Fitxers
Tipus de document
ArticleVersió
Versió publicadaData de publicació
Llicència de publicació
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/199042
Uncovering tau in wasteosomes (corpora amylacea) of Alzheimer's disease patients
Títol de la revista
Director/Tutor
ISSN de la revista
Títol del volum
Recurs relacionat
Resum
Brain corpora amylacea, recently renamed as wasteosomes, are polyglucosan bodies that appear during aging and some neurodegenerative conditions. They collect waste substances and are part of a brain cleaning mechanism. For decades, studies on their composition have produced inconsistent results and the presence of tau protein in them has been controversial. In this work, we reanalyzed the presence of this protein in wasteosomes and we pointed out a methodological problem when immunolabeling. It is well known that to detect tau it is necessary to perform an antigen retrieval. However, in the case of wasteosomes, an excessive antigen retrieval with boiling dissolves their polyglucosan structure, releases the entrapped proteins and, thus, prevents their detection. After performing an adequate pre-treatment, with an intermediate time of boiling, we observed that some brain wasteosomes from patients with Alzheimer's disease (AD) contained tau, while we did not detect tau protein in those from non-AD patients. These observations pointed the different composition of wasteosomes depending on the neuropathological condition and reinforce the role of wasteosomes as waste containers.
Matèries (anglès)
Citació
Citació
RIBA BAQUÉS, Marta, VALLE I MACIÀ, Jaume del, ROMERA, Clara, ALSINA PLANELLES, Raquel, MOLINA PORCEL, Laura, PELEGRÍ I GABALDÀ, Carme, VILAPLANA I HORTENSI, Jordi. Uncovering tau in wasteosomes (corpora amylacea) of Alzheimer's disease patients. _Frontiers in Aging Neuroscience_. 2023. Vol. 15, núm. 1110425. [consulta: 1 de febrer de 2026]. ISSN: 1663-4365. [Disponible a: https://hdl.handle.net/2445/199042]