Integrative taxonomy of land planarians (Platyhelminthes: Geoplanidae) from the Andean-Patagonian Forests from Argentina and Chile, with the erection of two new genera

Abstract

Our knowledge about the diversity of land planarians (Platyhelminthes: Geoplanidae) from the Andean-Patagonian Forests is scarce compared with other forested biomes. These cold-temperate forests are located in southern Chile and western Patagonia in Argentina, at the southern end of South America. Many species of land planarians from this region are known from descriptions based on a single or only a few specimens. Therefore, the finding of new material can reveal cryptic species, as well as anatomical or histological differences among specimens due to different maturation stages, physiological states, or intraspecific variation. In this paper, we focus on two geoplaninid species, Geoplana valdiviana and Amaga ruca, with the main goal of offering detailed re-descriptions of both species (previously known from Chilean Patagonia) from new material recently found in Argentinean Patagonia. In this contribution, we added new information on histological and anatomical features of these species. After assessing taxonomically relevant anatomical characters together with DNA sequence data (mitochondrial COI gene and nuclear 18S rRNA gene), we took three taxonomic decisions: (a) G. valdiviana was removed from Geoplana and re-allocated in the new genus Inakayalia; (b) Geoplana chanca and Geoplana tirua were also included in this new genus; and (c) A. ruca was split off from the genus Amaga and placed in the new monotypic genus Wallmapuplana. In addition, the known distribution of these planarian species was expanded to include the Argentinean portion of the Andean-Patagonian Forests.