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Accepted version

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2021-08-31

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Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/180031

Molecular Detection of Ralstonia solanacearum to Facilitate Breeding for Resistance to Bacterial Wilt in Potato

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https://doi.org/10.1007/978-1-0716-1609-3_18

Abstract

Potato bacterial wilt is caused by the devastating bacterial pathogen Ralstonia solanacearum. Quantitative resistance to this disease has been and is currently introgressed from a number of wild relatives into cultivated varieties through laborious breeding programs. Here, we present two methods that we have developed to facilitate the screening for resistance to bacterial wilt in potato. The first one uses R. solanacearum reporter strains constitutively expressing the luxCDABE operon or the green fluorescent protein (gfp) to follow pathogen colonization in potato germplasm. Luminescent strains are used for nondestructive live imaging, while fluorescent ones enable precise pathogen visualization inside the plant tissues through confocal microscopy. The second method is a BIO-multiplex-PCR assay that is useful for sensitive and specific detection of viable R. solanacearum (IIB-1) cells in latently infected potato plants. This BIO-multiplex-PCR assay can specifically detect IIB-1 sequevar strains as well as strains belonging to all four R. solanacearum phylotypes and is sensitive enough to detect without DNA extraction ten bacterial cells per mL in complex samples.The described methods allow the detection of latent infections in roots and stems of asymptomatic plants and were shown to be efficient tools to assist potato breeding programs.

Subject

Patatera, Malalties bacterianes, Genètica vegetal

Subject (English)

Potato, Bacterial diseases, Plant genetics

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Articles publicats en revistes (Genètica, Microbiologia i Estadística)

Citation

FERREIRA, V., et al. Molecular Detection of Ralstonia solanacearum to Facilitate Breeding for Resistance to Bacterial Wilt in Potato. Methods in Molecular Biology. 2021. Vol. 2354, num. 375-385. ISSN 1940-6029. [consulted: 11 of June of 2026]. Available at: https://hdl.handle.net/2445/180031

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