Carregant...
Tipus de document
ArticleVersió
Versió publicadaData de publicació
Llicència de publicació
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/221882
Basal association of a transcription factor favors early gene expression
Títol de la revista
Director/Tutor
ISSN de la revista
Títol del volum
Recurs relacionat
Resum
Responses to extracellular signals via Mitogen-Activated Protein Kinase (MAPK)
pathways control complex transcriptional programs where hundreds of genes are
induced at a desired level with a specific timing. Gene expression regulation is largely
encoded in the promoter of the gene, which harbors numerous transcription factor
binding sites. In the mating MAPK pathway of Saccharomyces cerevisiae, one major
transcription factor, Ste12, controls the chronology of gene expression necessary for
the fusion of two haploid cells. Because endogenous promoters encode a large diversity of Ste12 binding sites (PRE), we engineered synthetic promoters to decipher
the rules that dictate mating gene induction. Conformations of PRE dimers that allow
efficient gene expression were identified. The strength of binding of Ste12 to the
PRE and the distance of the binding sites to the core promoter modulate the level of
induction. The speed of activation is ensured by favoring a basal association of Ste12
by using a strong dimer of PRE located in a nucleosome depleted region.
Matèries (anglès)
Citació
Citació
PINHEIRO, Sandrine, NADAL RIBELLES, Mariona, SOLÉ SERRA, Carme, VINCENZETTI, Vincent, DUSSERRE, Yves, POSAS, Francesc, PELET, Serge. Basal association of a transcription factor favors early gene expression. _PLoS Genetics_. 2025. Vol. 21, núm. 6. [consulta: 23 de gener de 2026]. ISSN: 1553-7404. [Disponible a: https://hdl.handle.net/2445/221882]