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Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/221882
Basal association of a transcription factor favors early gene expression
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Responses to extracellular signals via Mitogen-Activated Protein Kinase (MAPK)
pathways control complex transcriptional programs where hundreds of genes are
induced at a desired level with a specific timing. Gene expression regulation is largely
encoded in the promoter of the gene, which harbors numerous transcription factor
binding sites. In the mating MAPK pathway of Saccharomyces cerevisiae, one major
transcription factor, Ste12, controls the chronology of gene expression necessary for
the fusion of two haploid cells. Because endogenous promoters encode a large diversity of Ste12 binding sites (PRE), we engineered synthetic promoters to decipher
the rules that dictate mating gene induction. Conformations of PRE dimers that allow
efficient gene expression were identified. The strength of binding of Ste12 to the
PRE and the distance of the binding sites to the core promoter modulate the level of
induction. The speed of activation is ensured by favoring a basal association of Ste12
by using a strong dimer of PRE located in a nucleosome depleted region.
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PINHEIRO, Sandrine, et al. Basal association of a transcription factor favors early gene expression. PLoS Genetics. 2025. Vol. 21, num. 6. ISSN 1553-7404. [consulted: 16 of October of 2026]. Available at: https://hdl.handle.net/2445/221882