Carregant...
Fitxers
Tipus de document
ArticleVersió
Versió acceptadaData de publicació
Llicència de publicació
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/115793
Biophysical characterization of the association of histones with single-stranded DNA
Títol de la revista
Director/Tutor
ISSN de la revista
Títol del volum
Recurs relacionat
Resum
BACKGROUND: Despite the profound current knowledge of the
architecture and dynamics of nucleosomes, little is known about
the structures generated by the interaction of histones with
single-stranded DNA (ssDNA), which is widely present during
replication and transcription. METHODS: Non-denaturing gel
electrophoresis, transmission electron microscopy, atomic force
microscopy, magnetic tweezers. RESULTS: Histones have a high
affinity for ssDNA at physiological salt concentrations, with an
apparent binding constant similar to that calculated for their
association with double-stranded DNA (dsDNA). The length of DNA
(number of nucleotides in ssDNA or base pairs in dsDNA)
associated with a fixed core histone mass is the same for both
ssDNA and dsDNA. Whereas histone-ssDNA complexes show a high
tendency to aggregate in 0.2 M NaCl, at lower ionic strength
nucleosome-like structures are formed. Core histones are able to
protect ssDNA from digestion by micrococcal nuclease, and a
shortening of ssDNA occurs upon its interaction with histones.
The purified (+) strand of a cloned DNA fragment of nucleosomal
origin has a higher affinity for histones than the purified
complementary (-) strand. CONCLUSIONS: At physiological ionic
strength histones have high affinity for ssDNA, possibly
associating with it into nucleosome-like structures. General
Significance In the cell nucleus histones may spontaneously
interact with ssDNA to facilitate their participation in the
replication and transcription of chromatin.
Matèries (anglès)
Citació
Col·leccions
Citació
WANG, Ying, MERWYK, Luis van, TÖNSING, Katja, WALHORN, Volker, ANSELMETTI, Dario, FERNÀNDEZ BUSQUETS, Xavier. Biophysical characterization of the association of histones with
single-stranded DNA. _Biochimica et Biophysica Acta. General Subjects_. 2017. [consulta: 25 de gener de 2026]. ISSN: 0006-3002. [Disponible a: https://hdl.handle.net/2445/115793]