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2017-07-27

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cc-by-nc-nd (c) Elsevier, 2017
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/115793

Biophysical characterization of the association of histones with single-stranded DNA

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http://dx.doi.org/10.1016/j.bbagen.2017.07.018

Abstract

BACKGROUND: Despite the profound current knowledge of the architecture and dynamics of nucleosomes, little is known about the structures generated by the interaction of histones with single-stranded DNA (ssDNA), which is widely present during replication and transcription. METHODS: Non-denaturing gel electrophoresis, transmission electron microscopy, atomic force microscopy, magnetic tweezers. RESULTS: Histones have a high affinity for ssDNA at physiological salt concentrations, with an apparent binding constant similar to that calculated for their association with double-stranded DNA (dsDNA). The length of DNA (number of nucleotides in ssDNA or base pairs in dsDNA) associated with a fixed core histone mass is the same for both ssDNA and dsDNA. Whereas histone-ssDNA complexes show a high tendency to aggregate in 0.2 M NaCl, at lower ionic strength nucleosome-like structures are formed. Core histones are able to protect ssDNA from digestion by micrococcal nuclease, and a shortening of ssDNA occurs upon its interaction with histones. The purified (+) strand of a cloned DNA fragment of nucleosomal origin has a higher affinity for histones than the purified complementary (-) strand. CONCLUSIONS: At physiological ionic strength histones have high affinity for ssDNA, possibly associating with it into nucleosome-like structures. General Significance In the cell nucleus histones may spontaneously interact with ssDNA to facilitate their participation in the replication and transcription of chromatin.

Subject

Histones, Cromatina

Subject (English)

Histones, Chromatine

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WANG, Ying, et al. Biophysical characterization of the association of histones with
                single-stranded DNA. Biochimica et Biophysica Acta. General Subjects. 2017. ISSN 0006-3002. [consulted: 15 of August of 2026]. Available at: https://hdl.handle.net/2445/115793

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