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Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/177193

Insuline-like effects of vanadate on glucokinase activity and fructose 2,6-bisphosphate levels in the liver of diabetic rats

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Streptozotocin diabetic rats showed more than a 4-fold increase in blood glucose levels, whereas hepatic glycogen, fructose 2,6-bisphosphate concentration, and 6-phosphofructo-2-kinase activity were decreased. The 'total' 6-phosphofructo-2-kinase and the 'active' (nonphosphorylated) form of the enzyme were decreased to a different extent, resulting in a fall of the 'active'/'total' activity ratio. Vanadate administration for a 2-week period restored the altered values in the diabetic rats without modifying significantly in the control animals any of the parameters studied. Glucokinase activity was essentially lacking in the diabetic animals, and vanadate treatment restored the activity to about 65% of its control value, a good correlation between the recovery of the enzyme and the blood glucose level being observed. These results show an insulin-like effect of vanadate in the whole animal and suggest that insulin and vanadate possess similar actions on hepatic intracellular events.

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GIL I SANTANO, Joan, et al. Insuline-like effects of vanadate on glucokinase activity and fructose 2,6-bisphosphate levels in the liver of diabetic rats. Journal of Biological Chemistry. 1988. Vol. 263, num. 4, pags. 1868-1871. ISSN 0021-9258. [consulted: 8 of August of 2026]. Available at: https://hdl.handle.net/2445/177193

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