Amb motiu del tancament d'estiu, la validació de documents es reprendrà a partir del 28 d'agost de 2026. Disculpeu les molèsties.
Con motivo del cierre de verano, la validación de documentos se reanudará a partir del 28 de agosto de 2026. Disculpad las molestias
Due to the summer closure, document validation will resume starting August 28, 2026. We apologize for any inconvenience.

Files

ARAÑÓ_ANSOLA_JAVIER_TFM.pdf (1.53 MB)

Document type

Master thesis

Publication date

2024-08-05

Publication license

cc-by-nc-nd (c) Arañó Ansola, Javier, 2024
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/216077

Assembling the largest 18S ribosomal RNA curated data set for non-bilaterian animals

Journal Title

Authors

Director/Tutor

Journal ISSN

Volume Title

Related resource

Abstract

The small subunit ribosomal RNA (SSU rRNA), named 18S in eukaryotes, is a gene universally present in all branches of the Tree of Life that has been instrumental to solve the most ancient relationships. Furthermore, it serves as a molecular identifier of biodiversity in environmental DNA studies. Despite its significance, specialised 18S databases still contain errors and struggle to keep pace with the rapidly changing eukaryotic taxonomy and the influx of novel diversity. This challenge hinders the assembly of reliable reference phylogenetic trees needed to identify novel 18S sequences obtained through metabarcoding studies. As part of a larger project aimed at creating reference databases for eukaryotic 18S (EukRef), this work focuses on curating the 18S sequences of non-bilaterian animals (sponges, ctenophores, cnidarians and placozoans) in the Protist Ribosomal Reference Database (PR2). Through this curation process, we generated the largest backbone phylogenetic tree for non-bilaterians, essential for the taxonomic identification of 18S sequences within this group. Furthermore, we reaffirmed that 18S is a suitable phylogenetic marker for animals, as it can recover most well-known clades to the phylum level. Lastly, we confirmed that incorporating 18S secondary structure information into sequence alignment positively impacts the topology of the inferred animal Tree of Life.

Description

Treballs Finals de Màster en Genètica i Genòmica. Facultat de Biologia. Universitat de Barcelona. Curs 2023-2024. Tutors/Directors: Jesus Lozano Fernandez Mattia, Mattia Giacomelli

Subject

Biologia molecular, Filogènia, Treballs de fi de màster

Subject (English)

Molecular biology, Phylogeny, Bachelor's theses

Citation

Collections

Màster Oficial - Genètica i Genòmica

Citation

ARAÑÓ ANSOLA, Javier. Assembling the largest 18S ribosomal RNA curated data set for non-bilaterian animals. [consulted: 18 of August of 2026]. Available at: https://hdl.handle.net/2445/216077

Export metadata

JSON - METS

Share record