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cc-by-nc (c) Bäuerl, Christine et al., 2021
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/184911

SARS-CoV-2 RNA and antibody detection in human milk from a prospective multicenter study in Spain

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Objectives To develop and validate a specific protocol for SARS-CoV-2 detection in breast milk matrix and to determine the impact of maternal SARS-CoV-2 infection on the presence, concentration and persistence of specific SARS-CoV-2 antibodies. Design and patients This is a prospective, multicentre longitudinal study (April-December 2020) in 60 mothers with SARS-CoV-2 infection and/or who have recovered from COVID-19. A control group of 13 women before the pandemic were also included. Setting Seven health centres from different provinces in Spain. Main outcome measures Presence of SARS-CoV-2 RNA in breast milk, targeting the N1 region of the nucleocapsid gene and the envelope (E) gene; presence and levels of SARS-CoV-2-specific immunoglobulins (Igs)¿IgA, IgG and IgM¿in breast milk samples from patients with COVID-19. Results All breast milk samples showed negative results for presence of SARS-CoV-2 RNA. We observed high intraindividual and interindividual variability in the antibody response to the receptor-binding domain of the SARS-CoV-2 spike protein for each of the three isotypes IgA, IgM and IgG. Main Protease (MPro) domain antibodies were also detected in milk. 82.9% (58 of 70) of milk samples were positive for at least one of the three antibody isotypes, with 52.9% of these positive for all three Igs. Positivity rate for IgA was relatively stable over time (65.2%-87.5%), whereas it raised continuously for IgG (from 47.8% for the first 10 days to 87.5% from day 41 up to day 206 post-PCR confirmation). Conclusions Our study confirms the safety of breast feeding and highlights the relevance of virus-specific SARS-CoV-2 antibody transfer. This study provides crucial data to support official breastfeeding recommendations based on scientific evidence.

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BÄUERL, Christine, RANDAZZO, Walter, SÁNCHEZ, Gloria, SELMA-ROYO, Marta, GARCIA-VERDEVIO, Elia, MARTÍNEZ RODRÍGUEZ, Laura, PARRA-LLORCA, Anna, LERIN, Carles, FUMADÓ, Victoria, CROVETTO, Francesca, CRISPI BRILLAS, Fàtima, PÉREZ-CANO, Francisco j., RODRÍGUEZ, Gerardo, RUÍZ-REDONDO, Gema, CAMPOY, Cristina, MARTÍNEZ COSTA, Cecilia, COLLADO, Maria carmen, MilkCORONA study team. SARS-CoV-2 RNA and antibody detection in human milk from a prospective multicenter study in Spain. _Archives of Disease in Childhood: Fetal & Neonatal_. 2021. Vol. 107, núm. 2, pàgs. 216-221. [consulta: 21 de gener de 2026]. ISSN: 1359-2998. [Disponible a: https://hdl.handle.net/2445/184911]

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