Carregant...
Fitxers
Tipus de document
ArticleVersió
Versió publicadaData de publicació
Tots els drets reservats
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/25624
Rheology of passive and adhesion-activated neutrophils probed by Atomic Force Microscopy
Títol de la revista
Director/Tutor
ISSN de la revista
Títol del volum
Recurs relacionat
Resum
The rheology of neutrophils in their passive and activated states plays a key role in determining their function in response to inflammatory stimuli. Atomic force microscopy was used to study neutrophil rheology by measuring the complex shear modulus G*(ω) of passive nonadhered rat neutrophils on poly(HEMA) and neutrophils activated through adhesion to glass. G*(ω) was measured over three frequency decades (0.1–102.4 Hz) by indenting the cells 500 nm with a spherical tip and then applying a 50-nm amplitude multi-frequency signal. G*(ω) of both passive and adhered neutrophils increased as a power law with frequency, with a coupling between elastic (G′) and loss (G″) moduli. For passive neutrophils at 1.6 Hz, G′ = 380 ± 121 Pa, whereas G″ was fourfold smaller and the power law coefficient was of x = 1.184. Adhered neutrophils were over twofold stiffer with a lower slope (x = 1.148). This behavior was adequately described by the power law structural damping model but not by liquid droplet and Kelvin models. The increase in stiffness with frequency may modulate neutrophil transit, arrest, and transmigration in vascular microcirculation.
Matèries
Matèries (anglès)
Citació
Citació
ROCA-CUSACHS SOULERE, Pere, ALMENDROS LÓPEZ, Isaac, SUNYER, Raimon, GAVARA I CASAS, Núria, FARRÉ VENTURA, Ramon, NAVAJAS NAVARRO, Daniel. Rheology of passive and adhesion-activated neutrophils probed by Atomic Force Microscopy. _Biophysical Journal_. 2006. Vol. 91, núm. núm 9, pàgs. 3508-3518. [consulta: 20 de gener de 2026]. ISSN: 0006-3495. [Disponible a: https://hdl.handle.net/2445/25624]