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2007

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Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/54926

Enrichment cultures should be performed in the detection of bacterial oral human pathogens in DUWLs

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Abstract

Water delivered by dental units during routine dental practice is densely contaminated by bacteria. The aim of this study was to determine actual isolation of the microorganisms sprayed from Dental Unit Water Lines (DUWLs) when enrichment cultures are performed and to compare frequencies with those obtained without enrichment cultures. Moreover, the antimicrobial susceptibilities of the microorganisms isolated were also studied. Water samples were collected from one hundred dental equipments in use at Dental Hospital of our University in order to evaluate the presence/absence of microorganisms and to perform their presumptive identification. Aliquots from all of the samples were inoculated in eight different media including both enrichment and selective media. Minimal inhibitory concentrations (MIC) were determined by the broth dilution method. The results herein reported demonstrate that most of the DUWLs were colonized by bacteria from human oral cavity; when enrichment procedures were applied the percentage of DUWLs with detectable human bacteria was one hundred percent. The results showed that in order to evaluate the actual risk of infections spread by DUWLs the inclusion of a step of pre-enrichment should be performed. The need for devices preventing bacterial contamination of DUWLs is a goal to be achieved in the near future that would contribute to maintain safety in dental medical assistance

Subject

Contaminació de l'aigua, Aparells i instruments odontològics, Bacteris patògens

Subject (English)

Water pollution, Dental instruments and apparatus, Pathogenic bacteria

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Articles publicats en revistes (Patologia i Terapèutica Experimental)

Citation

RUIZ, L., et al. Enrichment cultures should be performed in the detection of bacterial oral human pathogens in DUWLs. Bulletin du GIRSO. 2007. Vol. 48, num. 1, pags. 15-21. ISSN 0250-4693. [consulted: 12 of August of 2026]. Available at: https://hdl.handle.net/2445/54926

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