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2020

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cc-by-nc-nd (c) Elsevier B.V., 2020
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/151387

Improving separation optimization in capillary electrophoresis by using a general quality criterion

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Recurs relacionat

https://doi.org/10.1016/j.talanta.2019.120399

Resum

In this paper we extend the use of the quality criterion t' to optimize separations in capillary electrophoresis (CE). The theoretical parameter t' takes into account not only the relative separation between a given pair of compounds but also their separation from the neutral species migrating with the electroosmotic flow (EOF). Furthermore, it can be composed for complex mixtures as a global multicriterium optimization function T', for a rapid, simple and reliable selection of optimized separation conditions by mathematical maximization. Here, we demonstrate the applicability of T' using as a variable the electrophoretic mobility (me) for the optimization of pH in the separation of a mixture of amyloid beta (Aβ) peptide fragments. In addition, it is shown the versatility of T' using other variables related to me, which do not require experimental measurements. This is the case with ionizable compounds as the Aβ peptide fragments, whose charge-to-mass ratios can be calculated if accurate pKa values are available in the literature. The excellent performance of T' for Aβ peptide fragments is further validated optimizing the pH for the separation of mixtures of harmala alkaloids (HAlks) and quinolone antibiotics.

Matèries

Electroforesi capil·lar, Concentració dels ions d'hidrogen

Matèries (anglès)

Capillary electrophoresis, Hydrogen-ion concentration

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Col·leccions

Articles publicats en revistes (Enginyeria Química i Química Analítica)

Citació

PERÓ GASCÓN, Roger, TASCON, Marcos, SANZ NEBOT, María victoria, GAGLIARDI, Riccardo, BENAVENTE MORENO, Fernando j. (julián). Improving separation optimization in capillary electrophoresis by using a general quality criterion. _Talanta_. 2020. Vol. 208, núm. 120399. [consulta: 20 de gener de 2026]. ISSN: 0039-9140. [Disponible a: https://hdl.handle.net/2445/151387]

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