Carregant...
Fitxers
Tipus de document
ArticleVersió
Versió acceptadaData de publicació
Tots els drets reservats
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/17522
Development and application of a one-step low cost procedure to concentrate viruses from seawater samples
Títol de la revista
Director/Tutor
ISSN de la revista
Títol del volum
Recurs relacionat
Resum
A novel and simple procedure for concentrating adenoviruses from seawater samples is described. The technique entails the adsorption of viruses to pre-flocculated skimmed milk proteins, allowing the flocs to sediment by gravity, and dissolving the separated sediment in phosphate buffer. Concentrated virus may be detected by PCR techniques following nucleic acid extraction. The method requires no specialized equipment other than that usually available in routine public health laboratories, and due to its straightforwardness it allows the processing of a larger number of water samples simultaneously. The usefulness of the method was demonstrated in concentration of virus in multiple seawater samples during a survey of adenoviruses in coastal waters.
Matèries
Matèries (anglès)
Citació
Citació
CALGUA DE LEÓN, Byron thomas, MENGEWEIN, A., GRÜNERT, Andreas, BOFILL MAS, Silvia, CLEMENTE CASARES, Pilar, HUNDESA GONFA, Ayalkibet, WYN-JONES, A. peter, LÓPEZ-PILA, Juan, GIRONÈS LLOP, Rosina. Development and application of a one-step low cost procedure to concentrate viruses from seawater samples. _Journal of Virological Methods_. 2008. Vol. volumen 153, núm. 79-83. [consulta: 23 de gener de 2026]. ISSN: 0166-0934. [Disponible a: https://hdl.handle.net/2445/17522]