Files
Document type
ArticleVersion
Published versionPublication date
Publication license
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/191295
Membrane protein stabilization strategies for structural and functional studies
Journal Title
Director/Tutor
Journal ISSN
Volume Title
Related resource
Abstract
Accounting for nearly two-thirds of known druggable targets, membrane proteins are highly relevant for cell physiology and pharmacology. In this regard, the structural determination of pharmacologically relevant targets would facilitate the intelligent design of new drugs. The structural biology of membrane proteins is a field experiencing significant growth as a result of the development of new strategies for structure determination. However, membrane protein preparation for structural studies continues to be a limiting step in many cases due to the inherent instability of these molecules in non-native membrane environments. This review describes the approaches that have been developed to improve membrane protein stability. Membrane protein mutagenesis, detergent selection, lipid membrane mimics, antibodies, and ligands are described in this review as approaches to facilitate the production of purified and stable membrane proteins of interest for structural and functional studies.
Subject
Subject (English)
Citation
Citation
ERRASTI-MURUGARREN, Ekaitz, BARTOCCIONI, Paola and PALACÍN PRIETO, Manuel. Membrane protein stabilization strategies for structural and functional studies. Membranes. 2021. Vol. 11, num. 2. ISSN 2077-0375. [consulted: 12 of June of 2026]. Available at: https://hdl.handle.net/2445/191295