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2015-07-30

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cc-by (c) Solórzano, Carla et al., 2015
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/66705

Hha has a defined regulatory role that is not dependent upon H-NS or StpA

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Recurs relacionat

http://dx.doi.org/10.3389/fmicb.2015.00773

Resum

The Hha family of proteins is involved in the regulation of gene expression in enterobacteria by forming complexes with H-NS-like proteins. Whereas several amino acid residues of both proteins participate in the interaction, some of them play a key role. Residue D48 of Hha protein is essential for the interaction with H-NS, thus the D48N substitution in Hha protein abrogates H-NS/Hha interaction. Despite being a paralog of H-NS protein, StpA interacts with HhaD48N with higher affinity than with the wild type Hha protein. To analyze whether Hha is capable of acting independently of H-NS and StpA, we conducted transcriptomic analysis on the hha and stpA deletion strains and the hhaD48N substitution strain of Salmonella Typhimurium using a custom microarray. The results obtained allowed the identification of 120 genes regulated by Hha in an H-NS/StpA-independent manner, 38% of which are horizontally acquired genes. A significant number of the identified genes are involved in functions related to cell motility, iron uptake, and pathogenicity. Thus, motility assays, siderophore detection and intra-macrophage replication assays were performed to confirm the transcriptomic data. Our findings point out the importance of Hha protein as an independent regulator in S. Typhimurium, highlighting a regulatory role on virulence.

Matèries

Proteïnes, Salmonel·la, Genòmica

Matèries (anglès)

Proteins, Salmonella, Genomics

Citació

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Articles publicats en revistes (Genètica, Microbiologia i Estadística)

Citació

SOLÓRZANO, Carla, et al. Hha has a defined regulatory role that is not dependent upon H-NS or StpA. Frontiers in Microbiology. 2015. Vol. 6, num. 773-773. ISSN 1664-302X. [consulted: 20 of August of 2026]. Available at: https://hdl.handle.net/2445/66705

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