Protocol for tailored in vitro neuronal networks on high-density microelectrode arrays with polydimethylsiloxane microstructures

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dc.contributor.authorHaeb, Anna-Christina
dc.contributor.authorYamamoto, Hideaki
dc.contributor.authorRoach, Paul
dc.contributor.authorMerryweather, Daniel
dc.contributor.authorSato, Y.
dc.contributor.authorTornero, Daniel
dc.contributor.authorSoriano i Fradera, Jordi
dc.date.accessioned2026-03-18T08:32:26Z
dc.date.available2026-03-18T08:32:26Z
dc.date.issued2026-03-20
dc.date.updated2026-03-18T08:32:29Z
dc.description.abstractComplementary metal-oxide-semiconductor (CMOS)-based high-density microelectrode arrays (HD-MEAs) enable neuronal recordings with high spatiotemporal resolution. However, integrating polydimethylsiloxane (PDMS) microstructures onto HD-MEA surfaces to control network architecture is currently challenging and platform specific. Here, we present a protocol for PDMS fabrication, HD-MEA chip preparation, PDMS-HD-MEA microstructure alignment, and cell culture, including alternatives. Our results show reproducible formation of modular networks with characteristic activity patterns across different systems. This protocol supports engineering of defined neuronal architectures while maintaining compatibility with various HD-MEA systems.
dc.format.extent25 p.
dc.format.mimetypeapplication/pdf
dc.identifier.idgrec767037
dc.identifier.pmid41621071
dc.identifier.urihttps://hdl.handle.net/2445/228245
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.isformatofReproducció del document publicat a: https://doi.org/10.1016/j.xpro.2026.104349
dc.relation.ispartofSTAR protocols, 2026, vol. 7, num.1
dc.relation.urihttps://doi.org/10.1016/j.xpro.2026.104349
dc.rightscc-by (c) Haeb, Anna-Christina, et al., 2026
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.classificationSistemes microelectromecànics
dc.subject.classificationXarxes neuronals (Neurobiologia)
dc.subject.classificationMicroelectrònica
dc.subject.otherMicroelectromechanical systems
dc.subject.otherNeural networks (Neurobiology)
dc.subject.otherMicroelectronics
dc.titleProtocol for tailored in vitro neuronal networks on high-density microelectrode arrays with polydimethylsiloxane microstructures
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion

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