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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/176410
Generation of induced pluripotent stem cells from human cord blood using OCT4 and SOX2
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Mouse and human fibroblasts were the first cell types successfully reprog- rammed by ectopic expression of OCT4, SOX2, KLF4, and c-MYC (OSKM) (Lowry et al., 2008; Maherali et al., 2007; Park et al., 2008; Takahashi et al., 2007; Taka- hashi and Yamanaka, 2006; Yu et al., 2007). Further studies have shown that the age, origin, and cell type used have a deep impact on the reprogramming effi- ciency, eventually requiring the expres- sion of fewer factors and/or reducing the timing of the whole process. In general, stem cells are rare and difficult to access and isolate in large numbers (neural stem cells, for instance [Kim et al., 2008, 2009c]) and, therefore, represent a com- plicated target for reprogramming. How- ever, Cord Blood (CB) could represent an alternative and readily accessible source of stem cells. Here, we describe reprog- ramming of CB cells to pluripotency by retroviral transduction of four (OSKM), three (OSK), and as few as two (OS) tran- scription factors, without the need for additional chemical compounds.
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GIORGETTI, Alessandra, MONTSERRAT, Núria, AASEN, Trond, GONZALEZ, Federico, RODRÍGUEZ-PIZÀ, Ignacio, VASSENA, Rita, RAYA CHAMORRO, Ángel, BOUÉ, Stefanie, BARRERO, Maria j., ARAN CORBELLA, Begoña, TORRABADELLA, Marta, VEIGA, Anna, IZPISÚA BELMONTE, Juan carlos. Generation of induced pluripotent stem cells from human cord blood using OCT4 and SOX2. _Cell Stem Cell_. 2009. Vol. 5, núm. 4, pàgs. 353-357. [consulta: 20 de gener de 2026]. ISSN: 1934-5909. [Disponible a: https://hdl.handle.net/2445/176410]